Article

Efficacies of potential probiotic candidates isolated from traditional fermented Korean foods in stimulating immunoglobulin A secretion

Chang-Yong Choi1,, Chang-Hee Lee1,, Jun Yang1, Seok-Jin Kang1, In-Byung Park1, Si-Won Park1, Na-Young Lee1, Hyun-Been Hwang1, Hyun Sun Yun2, Taehoon Chun1,*
Author Information & Copyright
1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea.
2CJ CheilJedang Corporation, Suwon 16495, Korea.

† These authors contributed equally to this work.

*Corresponding Author: Taehoon Chun, E-mail: tchun@korea.ac.kr.

© Copyright 2023 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Nov 25, 2022 ; Revised: Jan 04, 2023 ; Accepted: Jan 06, 2023

Published Online: Jan 20, 2023

Abstract

The aim of this study was to evaluate efficacies of selected lactic acid bacteria (LAB) in inducing immunoglobulin A (IgA) secretion. Twenty-five different LAB isolated from traditional fermented Korean foods were characterized for their probiotic properties and screened to identify those that could stimulate lamina propria cells (LPCs) from Peyer’s patch to secret IgA in vitro. Among them,four strains (Lactiplantibacillus plantarum CJW55-10, Lactiplantibacillus pentosus CJW18-6, Lact. pentosus CJW56-11, and Pediococcus acidilactici CJN2696) were found to be strong IgA inducers. The number of IgA positive B cells and soluble IgA level were increased when LPCs were co-cultured with these LAB. Expression levels of toll-like receptor (TLR) such as TLR2 and TLR4 and secretion of interleuckin-6 (IL-6) were augmented in LPCs treated with these LAB. Further, we determined whether oral intake of these LAB enhanced IgA production in vivo. After one-week of daily oral administration, these LAB feed mice increased mucosal IgA and serum IgA. In conclusion, selected strains of LAB could induce systemic IgA secretion by activating lamina propria B cells in Peyer’s patch and oral intake of selected strains of LAB can enhance systemic immunity by inducing mucosal IgA secretion.

Keywords: interleukin-6; immunoglobulin A; lactic acid bacteria; Peyer’s patch; toll-like receptor