Physiological Characteristics and Anti-diabetic Effect of Pediococcus pentosaceus KI62
Received: Jul 28, 2020 ; Revised: Oct 29, 2020 ; Accepted: Dec 11, 2020
Published Online: Dec 21, 2020
The purpose of this study is to examine the physiological characteristics and anti-diabetic effects of P. pentosaceus KI62. The α-amylase and α-glucosidase inhibitory activity of P. pentosaceus KI62 was 94.86±3.30% and 98.59±0.52%, respectively. The amounts of short chain fatty acids (SCFA) in MRS broth containing 3% maltodextrin inoculated by P. pentosaceus KI62 were propionic acid 18.05 ± 1.85 mg/kg, acetic acid 1.12 ± 0.07 g/100 mL, and butyric acid 2.19 ± 0.061 g / kg. The amounts of medium chain fatty acids (MCFA) in MRS broth containing 3% maltodextrin inoculated by P. pentosaceus KI62 were C8 0.262±0.031 mg/kg, C10 0.279±0.021mg/kg, and C12 0.203±0.009mg/kg. Compared to sixteen antibiotics, P. pentosaceus KI62 had the highest sensitivity to penicillin-G and rifampicin, as well as the highest resistance to vancomycin and ampicillin. The strain also showed higher leucine arylamidase and valine arylamidase activities than other enzyme activities, but it did not produce β-glucuronidase which is carcinogenic enzymes. The survival rate of P. pentosaceus KI62 in 0.3% bile was 91.67%. Moreover, the strain showed a 98.63% survival rate in pH 2.0. P. pentosaceus KI62 exhibits resistance to Escherichia coli, Salmonella Typhimurium, Listeria monocytogenes and Staphylococcus aureus at rates of 29.41%, 38.10%, 51.72% and 50.47%, respectively. P. pentosaceus (23.31%) showed a similar adhesion ability to L. rhamnosus GG, the positive control (24.49%). These results show that P. pentosaceus KI62 has possibility as a probiotic with anti-diabetic effects.