ARTICLE

# Functional Quails Eggs using Enriched Spirulina during the Biosorption Process

Maryam Vejdani nia1, Mozhgan Emtyazjoo2,*, Mohammad Chamani3
1Faculty of Food Technology, Islamic Azad University, Tehran, Iran
2Department of Marine Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
3Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
*Corresponding author : Mozhgan Emtyazjoo, Department of Marine Science, North Tehran Branch, Islamic Azad University, Tehran, Iran Tel: +989122106883 E-mail: m_emtyazjoo@Iau-tnb.ac.ir

© Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jan 20, 2021 ; Revised: Jun 14, 2021 ; Accepted: Jul 28, 2021

Published Online: Jan 01, 2022

## Abstract

Spirulina platensis was included at 2.5%, 5%, and 7.5% of the diet as a phytobiotic either as a crude preparation or after enrichment by a biosorption process and fed to 126 Japanese quail and the eggs collected and examined for 6 weeks. Assessments were made of physical and chemical characteristics of the eggs. All treatments with added Spirulina increased unsaturated fatty acids and decreased saturated fatty acids with the largest responses for linolenic (omega 3) and oleic (omega 9) acids. The changes in fatty acids were greater with enriched than crude Spirulina. These results suggest that eggs from quail fed with Spirulina may have positive effects on human health.

Keywords: functional foods; enrichment; omega 3; Spirulina platensis; quail eggs

## Introduction

Functional foods are consumed as part of a regular diet and in addition to their nutritional efficiency, they have beneficial physiological effects or reduce the risk of chronic diseases (Adefegha, 2018; Ovando et al., 2018). Microalgae are one of the most interesting sources of functional foods (Benelhadj et al., 2016; Priyadarshani and Rath, 2012). Arthrospira (Spirulina platensis) are microscopic filamentous prokaryotes (Vaz et al., 2016). Until a few years ago, fish oil was the only major source of polyunsaturated fatty acids. But now S. platensis oil, which contains significant amounts of polyunsaturated fatty acids, is also one of the main sources of these fatty acids. Algae can produce polyunsaturated fatty acids, such as docosahexaenoic acid, eicosapentaenoic acid, arachidonic acid, and gamma-linoleic acid. Numerous studies have confirmed that Spirulina is cholesterol-free and rich in polyunsaturated fatty acids (especially gamma-linolenic acid) and this makes Spirulina suitable for the treatment and prevention of atherosclerosis, obesity and high blood pressure. Due to the direct effects of gamma-linolenic acid on the immune system and use in the treatment of many diseases, there has been a great interest in producing high concentrations of gamma-linoleic acid (Andrade, 2018). Numerous studies have been done on the enrichment of quail feed with the use of S. platensis (Boiago et al., 2019), lycopene (Sahin et al., 2008), fish oil (Kamely et al., 2016), vegetation such as liquor ice (Doğan et al., 2018), and rice bran (Gopinger et al., 2016), to enrich its eggs (Wang et al., 2007). One of the side effects of using antibiotics is the formation of antibiotic resistance. This has created a crisis in human health treatment and highlighted the need for a new generation of antibiotics. The use of foods and supplements that naturally contain antimicrobial compounds has reduced the use of antibiotics by animals and their negative impact on consumers. Phytobiotics are a viable alternative and S. platensis supplements have given good results in aquaculture, poultry feeding, and agriculture (Belay et al., 1996).

Quail eggs are a good source of nutrients for human health, with marked advantages over chicken eggs (Jeke et al., 2018). Human body can not synthesize essential fatty acids and they must be received through the diet, Accordingly, the aim of this study was to investigate the effect of the addition of fortified S. platensis on the amount of essential fatty acids, especially omega 3, in quail eggs as this could provide, a functional and practical food for the diet of today’s societies.

## Materials and Methods

Biosorption in Spirulina platensis alga

S. platensis was enriched with iron and zinc by growing in a medium with added minerals. Samples were taken during the first (M1=at the beginning of the maximum progressive growth phase, after 7 days of initial cultivation, minerals 5 h before harvest added to S. platensis algae) and the second phases (M2=The cultivate algae after entering the logarithmic phase. In both methods EDTA-FeNa·3H2O, ferric citrate, ZnSO4·7H2O, and CuSO4·5H2O were added at the rates of 13 mg/L, 0.0396 mg/L, 0.5994 mg/L, and 0.1998 mg/L respectively (Shahtoori, 2015).

Analysis of absorption in Spirulina platensis

To evaluate the iron and zinc absorption in algal samples, the ICP-OES simultaneous Arcos EOP model was used. First, each treatment was digested and prepared separately (Sinaei et al., 2018). For this purpose, 1 g of the sample was digested with nitric acid and oxygenated water in the microwave for two 10 min steps at 200°C and 800 W power and the sample was finally injected into the device. To improve the accuracy of the test, the blank sample (containing nitric acid and oxygenated water without the original sample) was also injected into the device and finally, the concentration of heavy metals and elements was calculated using the following equation:

$M\left(\text{μg/g}\right)=\left(C×V\right)/W$

Where M is the final concentration of the elements and heavy metals of the sample in μg/g, C is the concentration obtained from the device in μg/L, V is the final sample volume in l (0.025), W the primary sample weight for acid digestion in (g) (modified Saeid et al., 2013a).

Feed, animals, housing and sampling

Crude S. platensis and S. platensis enriched by biosorption were each added to the base feed (2.5%, 5%, and 5.7%) and fed to 126 female Japanese quails. There were three replicates of each of the seven treatments, with six chicks per experimental unit. All chicks were eared on the same environmental conditions (Abouelezz, 2017). Experimental diets based on corn and soybean meal were adjusted for growth period and laying period (Table 1) (Hajati and Zaghari, 2019). During the experiment, the quails had free access to water and food and the rations were flour. Lighting was 24 h until 35 d, this was then reduced to 22 h with 2 h of darkness and 1 h was then added every night until it reached 8 h darkness and 16 h lighting. Laying ails started from 47 d. The eggs were collected during the 3 periods of 15 d.

Table 1. Basic feed components
Type Growth period1) Laying period2)
Value based on (g/kg feed) Value based on (g/kg feed)
Corn 30 35
Soy 23 19
Oil 10 7
Phosphate 10 16
Carbonate 10 50
Mineral supplement 1 1.5
Vitamin supplements 1 1.5
Methionine 1 1.5
Lysine 0.5 0.6
Threonine 0.1 0.6
Acid fire - 0.6
Anti-coccidiosis 0.1 -
Salt 1 1.5

1) 0–35 days old.

2) 35 days old- end of the period.

Analysis of fatty acids

Egg samples were collected at the end of the last period for analysis of fatty acids. Extraction of yolk oil: The quail egg yolk was stored at –20°C and then dried in a Christ-freezing dryer model at –80°C for 24 h at 0.0026 m bar. Hexane was added and it was kept in the refrigerator for 24 h. Samples were centrifuged at around 5,000×g at 4°C and then filtered through filter paper under vacuum condition, then the extracted oil was methylated. Fatty acid methyl ester preparation: To methylate the samples, toluene and 0.5 M sodium methoxide were added to the extracted oil. It was put in a water bath at 50°C for half an hour. Glacial acetic acid and distilled water were used to neutralize the alkali, then hexane was added to the test tube and the contents of the tube were mixed by vortexing. The tube was kept static for a few minutes and two phases formed. The lower phase contains water and the upper phase contains hexane and fatty acid methyl ester. This step was repeated. Anhydrous sodium sulfate was used for dehydration. After filtration with a Whatman 41 filter paper, the samples were placed under the hood in a water bath at 70°C to reach a volume of 1–2 mL. Samples were injected into a gas chromatography device (GC), model Shimadzu (Wang et al., 2000). During this experiment, the type of column was WAX with FID detector, injection and detector temperature was 240°C, Oven program temperature started on 60°C for 2 min and increased to 200°C at 10°C/min. It was kept at this temperature for 1 min and then the temperature was increased until 230°C at 5°C/min and kept there for 15 min (Jafari et al., 2014).

Analysis of egg quality parameters

Egg samples were collected randomly for evaluation of qualitative parameters during the 3 periods of 15 d.

Metric parameters

Egg weight parameters (EW), shape index (SI), egg volume (EV), eggshell surface (SSA), albumen index (AI), hough unit (HU), internal quality unit (IQU) and yolk index (YI) were measured on the short and long axies of the eggs using a digital caliper. Yolk and albumen diameters were measured after breaking the eggs on a smooth glass surface using a digital caliper. Yolk and albumen elevation were also measured using a standing caliper.

1. The SI was calculated as SI=d/D×100 with d and D representing the short axis and the long axis, respectively.

2. The EV was calculated as EV=4/3×π×(D/2)×(d/2)2 in which D=long egg axis, d=short egg axis and π=3.14159.

3. SSA was calculated as SSA=4.835×EW0.662 the equation below where EW=egg weight.

4. The AI was calculated as AI=h/(0.5×(D+d)) with h=height of concentrated albumen at the junction with yolk, D and d, are the long and short diameters of albumen, respectively.

5. HU was calculated as HU=100×log(h+7·57−1·7×EW0·37) with h=height of concentrated albumen at the junction with yolk and EW was=egg weight.

6. The internal quality of the egg (IQU) was calculated as IQU=100×log(h+4·18−0·89897×EW0·6674) with h=height of concentrated albumen at the junction with yolk and EW=egg weight.

7. Yolk quality was calculated by the YI as YI=h/D with h=yolk height and D=yolk diameter (Zita et al., 2013).

Eggshell strength and eggshell thickness

Eggshell strength parameters (ESS) and eggshell thickness (EST) were determined using egg multi tester model EMT-5200. For this purpose, Device Egg Shell Force Gauge Model-2 was used to measure the strength and Echo meter, 1061 (D-56 Wuppertal 1) was used to measure thickness (Abdanan Mehdizadeh et al., 2014).

Yolk color

In order to evaluate the yolk color (YC) and to ensure the accuracy of the test, assessments were made in all periods using the Roche color fan (0–15 degrees) (Ludke et al., 2018). In addition for eggs from the final 15-d period an assessment was made by Hunter Lab using Ultra scan VIS model (Carson et al., 1994).

Measuring the egg albumen pH

For this purpose, samples were collected in each of the three periods to measure the pH, the yolk was first separated from albumen, homogenized and measured with the pH meter of the NACI model (Doğan et al., 2018).

Measurement of moisture content

Egg samples from each of the three periods were weighed before and after the oven drying at 60°C for 72 h and moisture content of the samples was calculated (Gopinger et al., 2016).

Analysis of mineral absorption

Iron and zinc contents were analyzed in eggs from the final period and in algal samples.

Statistical analysis

A two-way analysis of variance was carried out using SPSS software version 22. The differences between the means of different treatments and time periods were considered significant at the 5% probability level. The data were normalized, before being tested statistically. Excel was used to draw charts.

## Results and Discussion

Absorption in Spirulina platensis

The amount of iron and zinc in the algae enriched in two ways, during the logarithmic phase (M2) and the maximum stationary phases (M1) shows in Fig. 1.

Fig. 1. Spirulina platensis changes during the enrichment process. The same name letters on the bar of each sample indicate no significant differences between the samples and the inconsistent letters indicate the significance of the samples with each other (p<0.05).

The amount of iron and zinc in the three groups differed significantly (p<0.05). These results indicate that the most suitable method for increasing iron and zinc contents in S. platensis algae is biosorption (method M2), with the increases being particularly large for zinc according to the results of Saeid et al. (2013a), the best method for enriching S. platensis is bioremediation, which is consistent with the results of this study.

Enrichment of eggs with enriched and crude Spirulina
Egg yolk fatty acids

The peak of fatty acids from gas chromatography of quail eggs fed with enriched, raw, and controlled algae shows in Fig. 2. Statistical results showed a significant difference (p<0.05) in saturated fatty acids (palmitic acid and stearic acid) and unsaturated fatty acids (oleic acid and linolenic acid) between treatments compared to the control group. In general consumption of crude and enriched S. platensis (M2) showed a significant difference (p<0.05) in the amount of saturated fatty acids (palmitic and stearic acid) and polyunsaturated fatty acids (oleic and linolenic acid) relative to the control group. According to these results both crude and enriched S. platensis were effective in reducing the saturated fatty acids of palmitic acid and stearic acid. The treatments 7.5 enrich, 2.5 enrich and 7.5 crude had the largest reduction compared to the control group for palmitic acid. However, the treatments crude 2.5 and enrich 5 did not show a significant decrease in palmitic acid compared to the control group, indicating a decrease in palmitic acid in more than 66% of the enrich and 33% of crude treatments, respectively. The results showed that stearic acid showed a significant decrease in all treatments, especially enriched treatments. The effect of feeding crude and enriched S. platensis (M2) on unsaturated fatty acids of oleic acid and linolenic acid was also significant, although 66% of treatments showed no increase in oleic acid content compared to control group, the amount of oleic acid in the enriched treatments 7.5 and 2.5 showed a significant increase compared to the control group. Also, linolenic acid showed the highest increase only in the crude treatment 5 compared to the control group. Due to reduced palmitic and stearic fatty acids and increased, oleic and linolenic fatty acids when S. platensis, especially in the enriched form, one would expect reductions in lower-density lipoprotein (LDL) and the risk of cardiovascular disease and stroke. According to the results, consumption of S. platensis did not show a significant effect on the amount of linoleic acid in the treated yolk compared to the control group. Boiago et al. (2019) reported that consumption of S. platensis at levels (0%, 5%, 10%, and 15%) for 42 days (2 21-day cycles) in quail feed, reduced saturated fatty acids and increased monounsaturated fatty acids while it decreased polyunsaturated fatty acids. Abouelezz (2017) reported that consumption of S. platensis at 1% level in Japanese quail feed reduced free fatty acids, also use of S. platensis at levels [2.5%, 5%, and 7.5%] for 12 weeks in rainbow trout feed increased some acid levels (Jafari et al., 2014).

Fig. 2. Chromatographs obtained by gas chromatographic analysis of quail egg yolk samples. A, with 2.5% of enriched Spirulina platensis; B, with 5% of enriched S. platensis; C, with 7.5% of enriched S. platensis; D, with 2.5% of crude S. platensis; E, with 5% of crude S. platensis; F, with 7.5% of crude S. platensis; G, Control.
Metric parameters of eggs

The results of Table 2 shows that S. platensis (M2) had a significant effect on egg quality parameters as assessed during the final period (EW, IQU, SI, EV, SSA, AI, and YI) (p<0.05). So that in EW, EV, and SSA parameters, 33.3% of treatments, SI of 50% of treatments (especially enrich treatments), IQU 83.3% of treatments (especially enrich treatments) and albumen and yolk indices The effects of S. platensis (M2) on egg quality parameters in samples taken in each of the three periods is shown in Fig. 3 the parameters (IQU, SSA, and EW) showed significant differences (p<0.05) between the three periods, so that all three parameters in the second period of the first period and in the third period It was longer than the first and second periods. There were significant differences (p<0.05) between periods in the HU showing decrease in albumen quality in the control group and better quality retention in egg albumen with addition of either crude or enriched S. platensis (M2) and crude compared with the control group. Dogan et al. (2016) reported that feeding 0.5%, 1%, 2% of S. platensis to quail for 8 weeks significantly increased albumen and yolk indices (p<0.05) and had a non-significant effect on HU (These results are in line with the results of this study. The present study showed that consumption of crude and enriched S. platensis had a significant effect on egg shape and weight index compared to the control group, while the results of Dogan et al. (2016) showed a significant effect (p<0.05) on egg production, but did not assess egg shape and weight index. In the present study, it was shown that consumption of crude and enriched S. platensis had a significant effect on EW, SI, and SSA in comparison to the control group, whereas Hajati and Zaghari (2019) found that consuming S. platensis at levels of 2.5, 5, 10, and 20 g/kg diet for 1 to 35 days and at levels of 1, 3, and 5 g/kg diet for up to 12 weeks gave decreases in these parameters.

Table 2. Effect of Spirulina platensis on the qualitative parameters of eggs collected in the first period
Parameter Crude 2.5% Crude 5% Crude 7.5% Enrich 2.5% Enrich 5% Enrich 7.5% Control
Egg weight (g) 11.09±0.9ab 11.13±0.48ab 12.85±1.9c 10.80±2.02a 12.78±1.8bc 11.42±1.7abc 11.75±1.6 abc
Shell thickness (mm) 22.52±1.6a 23.25±1.4a 23.52±1.4a 23.02±1.7a 23.00±1.5a 22.58±0.7a 22.55±1.5a
Shell strength (kg/cm2) 1.10±0.15a 1.24±0.34a 1.37±0.22a 1.09±0.4a 1.12±0.41a 1.19±0.14a 1.12±0.39a
Shape index (mm) 78.32±2.18a 77.95±1.74a 82.15±2.74b 80.30±2.5ab 78.65±2.11a 80.29±0.96ab 79.52±2.5a
Egg volume (cm3) 10.61±0.98a 11.12±0.6a 14.47±1.4c 10.96±1.2a 13.13±0.88b 11.19±0.5a 12.74±1.05b
Eggshell surface (cm2) 23.76±1.3ab 23.82±0.69ab 26.15±2.7b 23.27±2.9a 26.06±2.5b 24.18±2.4ab 24.66±2.2ab
Albumen index 0.1206±0.01a 0.1249±0.003 a 0.1186±0.01a 0.1267±0.01a 0.1203±0.01a 0.1413±0.02b 0.1184±0.01a
Hough unit 91.65±2.89a 89.11±2.89a 89.33±2.89a 91.84±2.8a 90.62±2.81a 91.55±3.82a 89.46±2.89a
Internal quality unit 65.36±5.8b 60.73±1.86ab 58.35±7.01a 65.95±6.6b 61.18±5.89ab 64.62±7.75ab 59.80±6ab
Yolk index 0.4644±0.02abc 0.4798±0.009bc 0.4590±0.01ab 0.4877±0.03c 0.4669±0.02abc 0.4441±0.01a 0.4421±0.03a

The same name letters above the numbers of each sample indicate no significant differences between the samples and the inconsistent letters indicate the significance of the samples with each other (p<0.05).

Fig. 3. Quality parameters of eggs during the three periods. IQU, internal quality unit; HU, hough unit; SSA, eggshell surface; EV, egg volume; SI, shape index; EW, egg weight; YC, yolk color; AI, albumen index.
Yolk color

The results of the effect of S. platensis on YC evaluated by Roche color fan showed a significant difference between treatment (p<0.05; Fig. 4). All treatments, especially with enriched Spirulina, showed a significant difference from the control group. Also, the evaluation of YC by Hunter’s lab showed a significant (p<0.05) effect of enriched S. platensis on lightness index (L*) and redness index (a*). With crude Spirulina a* increased and L* decreased. The highest redness was observed in the enriched 7.5 treatment and the highest transparency in the control group. This could be due to the presence of more iron in the enriched treatments and presence of pectin in Spirulina. The yellowness index (b*) did not show a significant difference (p>0.05) among the treatments, but the control group had a numerically higher b* than the other treatments. Hajati and Zaghari (2019) indicate an increase in YC, which is consistent with the results of this study. The results of yolk colorimetry are also consistent with the reports of (Omri et al., 2019), in which S. platensis was fed at 1.5% and 2.5% to 44-week-old chickens.

Fig. 4. Yolk color evaluation by Roche fan and Hunter lab.
Egg moisture and albumen pH

The effects of S. platensis on egg moisture and albumen pH are shown in Table 3. Statistical results indicated that there were no significant differences between the three periods in egg moisture content (p>0.05). However, the moisture content for the crude 5 treatments was significantly lower than that for all other treatments (p>0.05). Evaluation of albumen pH of samples showed no significant (p>0.05) statistical differences between treatments or periods, but there was a significant interaction between treatment in three periods (p<0.05), The lowest pH was for the 2.5% enriched sample in the first period and the highest pH for the control group in the third period.

Table 3. Changes in moisture factors and pH of eggs
Parameter Time period (15 day) Treatments
First period Second period Third period Crude 2.5% Crude 5% Crude 7.5% Enrich 2.5% Enrich 5% Enrich 7.5% Control
Moisture 70.19± 1.97a 70.36± 2.60a 70.64± 1.20a 70.09± 1.95b 68.53± 2.21a 71.02± 1.5b 70.48± 0.78b 71.24± 2.9b 70.84± 1.22b 70.59± 1.11b
pH 0.9193± 0.078a 0.9203± 0.004a 0.9213± 0.005a 0.922± 0.002a 0.9214± 0.002a 0.9196± 0.006a 0.9166± 0.011a 0.9208± 0.003a 0.919± 0.006a 0.9225± 0.002a

The same name letters above the numbers of each sample indicate no significant differences between the samples and the inconsistent letters indicate the significance of the samples with each other (p<0.05).

Absorption of minerals in quail eggs

The effect of enriched and crude S. platensis on iron and zinc contents of eggs are shown in Fig. 5. Eggs of quail fed enriched or crude algae generally showed higher iron and zinc contents than those from the control group, but there were some inconsistencies in the results and further research is required. The results do, however, indicate that enriched quail eggs could be a suitable food to ameliorate iron and zinc deficiency diseases in humans. Iron depletion in the 7.5% enriched treatment and higher absorption of this element in the crude treatment could be due to the antagonistic effect of iron with copper, iron with zinc or result from using an inappropriate source of the elements (they were supplied in inorganic form) with the results of Saeid et al. (2013b) study also indicate antagonistic effects of zinc with copper, zinc with iron, or inappropriate complement form when copper-enriched Spirulina was used in pig feed, A 2.5% enriched treatment gave increases in the absorption of iron, copper, chromium, selenium, manganese, but reduction in zinc.

Fig. 5. The amount of iron and zinc in eggs of quail fed with Spirulina platensis. The same name letters on the bar of each sample indicate no significant differences between the samples and the inconsistent letters indicate the significance of the samples with each other (p<0.05).

## Conclusion

Due to the role of essential fatty acids in the improvement and prevention of cardiovascular disease and bad cholesterol (LDL), the aim in this study was to produce a product enriched with essential fatty acids, especially omega-3. By including S. platensis in the diet we succeeded in producing quail eggs with 23% more linolenic acid (omega 3) than with the control group. The content of oleic acid was increased by 7.1%, whilst contents of palmitic and stearic saturated fatty acids were decreased significantly by 6.8% and 7.2%, respectively. This indicates considerable potential for the use of Spirulina to produce quail eggs with improved quality in relation to human health.

## Conflicts of interest

The authors declare no potential conflict of interest.

## Author Contributions

Conceptualization: Emtyazjoo M. Formal analysis: Vejdani nia M. Methodology: Vejdani nia M, Emtyazjoo M, Chamani M. Software: Vejdani nia M. Investigation: Vejdani nia M, Emtyazjoo M. Writing - original draft: Vejdani nia M, Emtyazjoo M. Writing – review & editing: Vejdani nia M, Emtyazjoo M, Chamani M.

## Ethics Approval

All animal studies have been approved by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards of the Islamic Azad University, and the experiment performed with respect to the International Guidelines for research involving animals (Directive 2010/63/EU).

## References

1.

Abdanan Mehdizadeh S, Minaei S, Hancock NH, Karimi Torshizi MA. 2014; An intelligent system for egg quality classification based on visible-infrared transmittance spectroscopy. Inf Process Agric. 1:105-114

2.

Abouelezz FMK. 2017; Evaluation of Spirulina algae (Spirulina platensis) as a feed supplement for Japanese quail: Nutritional effects on growth performance, egg production egg quality blood metabolites sperm-egg penetration and fertility. Egypt Poult Sci J. 37:707-719

3.

Adefegha SA. 2018; Functional foods and nutraceuticals as dietary intervention in chronic diseases; novel perspectives for health promotion and disease prevention. J Diet Suppl. 15:977-1009

4.

Andrade LM. 2018; Chlorella and spirulina microalgae as sources of functional foods, nutraceuticals, and food supplements; an overview. MOJ Food Process Technol. 6:00144

5.

Belay A, Kato T, Ota Y. 1996; Spirulina (Arthrospira): Potential application as an animal feed supplement. J Appl Phycol. 8:303-311

6.

Benelhadj S, Gharsallaoui A, Degraeve P, Attia H, Ghorbel D. 2016; Effect of pH on the functional properties of Arthrospira (Spirulina) platensis protein isolate. Food Chem. 194:1056-1063

7.

Boiago MM, Dilkin JD, Kolm MA, Barreta M, Souza CF, Baldissera MD, dos Santos ID, Wagner R, Tavernari FC, da Silva MLB, Zampar A, Stivanin TE, da Silva AS. 2019; Spirulina platensis in Japanese quail feeding alters fatty acid profiles and improves egg quality: Benefits to consumers. J Food Biochem. 43e12860

8.

Carson KJ, Collins JL, Penfield MP. 1994; Unrefined, dried apple pomace as a potential food ingredient. J Food Sci. 59:1213-1215

9.

Dogan SC, Baylan M, Erdogan Z, Akpinar GC, Kucukgul A, Duzguner V. 2016; Performance, egg quality and serum parameters of Japanese quails fed diet supplemented with Spirulina platensis. Fresenius Environ Bull. 25:5857-5862.

10.

Doğan SC, Yaman S, Baylan M, Bulancak A. 2018; Effects of dietary licorice root (Glycyrrhriza glabra) supplementation, storage time and temperature on quality of quail eggs. Prog Nutr. 20:665-671

11.

Gopinger E, Bavaresco C, Ziegler V, Lemes JS, Lopes DCN, Elias MC, Xavier EG. 2016; Performance, egg quality, and sensory analysis of the eggs of quails fed whole rice bran stabilized with organic acids and stored for different amounts of time. Can J Anim Sci. 96:128-134

12.

Hajati H, Zaghari M. 2019; Effects of Spirulina platensis on growth performance, carcass characteristics, egg traits and immunity response of Japanese quails. Iran J Appl Anim Sci. 9:347-357.

13.

Jafari SMA, Rabbani M, Emtyazjoo M, Piryaei F. 2014; Effect of dietary Spirulina platensis on fatty acid composition of rainbow trout (Oncorhynchus mykiss) fillet. Aquacult Int. 22:1307-1315

14.

Jeke A, Phiri C, Chitindingu K, Taru P. 2018; Ethnomedicinal use and pharmacological potential of Japanese quail (Coturnix coturnix japonica) birds meat and eggs, and its potential implications on wild quail conservation in Zimbabwe: A review. Cogent Food Agric. 4:1507305

15.

Kamely M, Karimi Torshizi MA, Khosravinia H. 2016; Omega-3 enrichment of quail eggs: Age, fish oil, and savory essential oil. J Agric Sci Technol. 18:347-359.

16.

Ludke MCMM, Pimentel ACS, Ludke JV, Silva JCNS, Rabello CBV, Santos JS. 2018; Laying performance and egg quality of Japanese quails fed diets containing castor meal and enzyme complex. Braz J Poult Sci. 20:781-788

17.

Omri B, Amraoui M, Tarek A, Lucarini M, Durazzo A, Cicero N, Santini A, Kamoun M. 2019; Arthrospira platensis (Spirulina) supplementation on laying hens’ performance: Eggs physical, chemical, and sensorial qualities. Foods. 8:386

18.

Ovando CA, Carvalho JC, Vinícius de Melo Pereira G, Jacques P, Soccol VT, Soccol CR. 2018; Functional properties and health benefits of bioactive peptides derived from Spirulina: A review. Food Rev Int. 34:34-51

19.

Priyadarshani I, Rath B. 2012; Commercial and industrial applications of micro algae: A review. J Algal Biomass. 3:89-100.

20.

Saeid A, Chojnacka K, Korczyński M, Korniewicz D. Dobrzański Z. 2013a. Effect on supplementation of Spirulina maxima enriched with Cu on production performance, metabolical and physiological parameters in fattening pigs. J Appl Phycol. 25:1607-1617

21.

Saeid A, Chojnacka K, Korczyński M, Korniewicz D, Dobrzański Z. 2013b; Biomass of Spirulina maxima enriched by biosorption process as a new feed supplement for swine. J Appl Phycol. 25:667-675

22.

Sahin N, Akdemir F, Orhan C, Kucuk O, Hayirli A, Sahin K. 2008; Lycopene-enriched quail egg as functional food for humans. Food Res Int. 41:295-300

23.

Shahtoori PM, Emtyazjoo M, Bonakdar S, Rabani M. 2015; The anticancer effects of hexane extract of rainbow trout (Oncorhynchus mykiss) muscle enriched with vegetable oil Brassica sp. Cancer Res J. 3:63-67

24.

Sinaei M, Loghmani M, Bolouki M. 2018; Application of biomarkers in brown algae (Cystoseria indica) to assess heavy metals (Cd, Cu, Zn, Pb, Hg, Ni, Cr) pollution in the northern coasts of the Gulf of Oman. Ecotoxicol Environ Saf. 164:675-680

25.

Vaz BD, Moreira JB, Morais MG, Costa JAV. 2016; Microalgae as a new source of bioactive compounds in food supplements. Curr Opin Food Sci. 7:73-77

26.

Wang L, Pan B, Sheng J, Xu J, Hu Q. 2007; Antioxidant activity of Spirulina platensis extracts by supercritical carbon dioxide extraction. Food Chem. 105:36-41

27.

Wang Y, Sunwoo H, Cherian G, Sim JS. 2000; Fatty acid determination in chicken egg yolk: A comparison of different methods. Poult Sci. 79:1168-1171

28.

Zita L, Ledvinka Z, Klesalová L. 2013; The effect of the age of Japanese quails on certain egg quality traits and their relationships. Vet Arh. 83:223-232.