Short Communication

Integrative analysis of probiotic-mediated remodeling in canine gut microbiota and metabolites using a fermenter for an intestinal microbiota model

Anna Kang1, Min-Jin Kwak1, Hye Jin Choi1, Seon-hui Son1, Sei-hyun Lim1, Ju Young Eor1, Minho Song2, Min Kyu Kim2, Jong Nam Kim3, Jungwoo Yang4, Minjee Lee4, Minkyoung Kang5, Sangnam Oh5,*, Younghoon Kim1,*
Author Information & Copyright
1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
2Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
3Department of Food Science & Nutrition, Dongseo University, Busan 47011, Korea.
5IBS R&D Center, Ildong Bioscience, Pyeongtaek-si 17957, Korea.
6Department of Functional Food and Biotechnology, Jeonju University, Jeonju 55069, Korea.
*Corresponding Author: Sangnam Oh. E-mail:
*Corresponding Author: Younghoon Kim. E-mail:

© Copyright 2024 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Nov 17, 2023 ; Revised: May 10, 2024 ; Accepted: May 10, 2024

Published Online: May 22, 2024


In this study, the FIMM system was employed to explore the interactions between intestinal microbiota and probiotics within a simulated gut environment. Two strains of commercial probiotic bacteria, Enterococcus faecium IDCC2102 and Bifidobacterium lactis IDCC4301, along with a newly isolated strain from domestic dogs, Lactobacillus acidophilus SLAM AK001, were introduced into the FIMM system in conjunction with the gut microbiota from a beagle model. Findings underscore the system's capacity to effectively mirror and modulate the gut environment, evidenced by a marked increase in beneficial bacteria like Lactobacillus and Faecalibacterium and a decrease in the opportunistic pathogen Clostridium. This study also verified the system's ability to facilitate accurate interactions between probiotics and commensal bacteria, demonstrated by the expected production of short-chain fatty acids and critical bacterial metabolites, including amino acids and GABA precursors. Thus, the results advocate for the application of FIMM as an in vitro cultivation system that authentically simulates the intestinal environment, presenting a viable alternative for examining the dynamics of gut microbiota and metabolites in companion animals

Keywords: in vitro culturomics; lactic acid bacteria; canines; FIMM; microbiome