Effect of bacteriocin-like inhibitory substance (BLIS) from Enterococcus faecium DB1 on cariogenic Streptococcus mutans biofilm formation

Ni-Na Kim¹, Bong Sun Kim¹, Han Bin Lee¹, Sunghyun An¹, Donghan Kim¹, Seok-Seong Kang¹^,^*

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¹Department of Food Science and Biotechnology, Dongguk University, Goyang 10326, Korea.

^*Corresponding Author: Seok-Seong Kang, Department of Food Science and Biotechnology, Dongguk University, Goyang 10326, Korea. E-mail: sskang@dgu.ac.kr.

© Copyright 2022 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jun 15, 2022 ; Revised: Aug 16, 2022 ; Accepted: Aug 22, 2022

Published Online: Sep 06, 2022

Abstract

The aim of the study was to investigate the effect of bacteriocin-like inhibitory substance (BLIS) from Enterococcus faecium DB1 on cariogenic Streptococcus mutans biofilm. Crystal violet staining, fluorescence, and scanning electron microscopy analyses demonstrated that the BLIS from Enterococcus faecium DB1 (DB1 BLIS) inhibited S. mutans biofilm. When DB1 BLIS was co-incubated with S. mutans, biofilm formation by S. mutans was significantly reduced (P < 0.05). DB1 BLIS also destroyed the preformed biofilm of S. mutans. In addition, DB1 BLIS decreased the viability of S. mutans biofilm cells during the development of biofilm formation and in the preformed biofilm. DB1 BLIS significantly decreased the growth of S. mutans planktonic cells. Furthermore, S. mutans biofilm on the surface of saliva-coated hydroxyapatite discs was reduced by DB1 BLIS. Taken together, DB1 BLIS might be useful as a preventive and therapeutic agent against dental caries caused by S. mutans.

Keywords: Enterococcus faecium; Bacteriocin-like inhibitory substance; Streptococcus mutans; biofilm; dental caries