Article

Prebiotics/Probiotics Mixture Induced Changes in Cecal Microbiome and Intestinal Morphology Alleviated Constipation in Mice

Min Guk Kim1,, Kyungae Jo1,, Kyoungwon Cho2, Sung Sun Park2, Hyung Joo Suh1,3,*, Ki-Bae Hong4,*
Author Information & Copyright
1Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea
2Chong Kun Dang Healthcare Co., Seoul 04300, Korea
3Transdisciplinary Major in Learning Health Systems, Department of Healthcare Sciences, Graduate 10 School, Korea University, Seoul 02841, Korea
4Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea

† These authors contributed equally to this work.

*Corresponding Author: Hyung Joo Suh, Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea. E-mail: suh1960@korea.ac.kr.
*Corresponding Author: Ki-Bae Hong, Jeju National University, Jeju 63243, Korea. E-mail: kbhong@jejunu.ac.kr.

© Copyright 2021 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 12, 2021 ; Revised: Mar 23, 2021 ; Accepted: Mar 23, 2021

Published Online: Mar 26, 2021

Abstract

The aim of this study was to investigate the effect of a mixture of multi-strain probiotics and prebiotics on loperamide-induced constipation in Sprague-Dawley rats. A multi-strain probiotic alone (loperamide-induced group with multi-strain probiotic mixture group; Lop-Pro) and a mixture of multi-strain probiotics and prebiotics (loperamide-induced group with multi-strain probiotic and prebiotic mixture group; Lop-Pro/Pre) were administered orally after inducing constipation. The fecal water content was significantly higher (by 42%) in the Lop-Pro/Pre group (33.5%) than in the loperamide-induced group (Lop) (23.7%) (p<0.05). The intestinal mucosal thickness, crypt cell area, and interstitial cells of Cajal area were significantly higher in the Lop-Pro/Pre group compared to the Lop group by 16.4%, 20.6%, and 42.3%, respectively. Additionally, the total short-chain fatty acid content was significantly increased in the Lop-Pro and Lop-Pro/Pre groups by 56.4% and 54.2%, respectively, compared with the Lop group. The Lop-Pro and Lop-Pro/Pre groups recovered loperamide-induced alteration in Bacteroidetes and Verrucomicrobia abundance among intestinal microbiota, whereas the Lop-Pro/Pre group recovered Akkermansia, Lactobacillus, Clostridium, Bacteroides, and Oscillibacter abundance. Moreover, the relative abundance of Oscillibacter and Clostridium was significantly different in the Lop-Pro/Pre group compared to the Lop group. Collectively, administration of synbiotics rather than multi-strain probiotics alone is effective in alleviating constipation.

Keywords: constipation; multi-strain probiotics; loperamide; microbiota


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