Article

Anti-biofilm effect of egg yolk phosvitin by inhibition of biomass production and adherence activity against Streptococcus mutans

Hyeon Joong Kim1, Jae Hoon Lee1, Dong Uk Ahn2, Hyun-Dong Paik1,*
Author Information & Copyright
1Konkuk University, Seoul 05029, Korea.
2Iowa State University, Ames 50011, United States.
*Corresponding Author: Hyun-Dong Paik, Konkuk University, Seoul 05029, Korea, Republic of. E-mail: hdpaik@konkuk.ac.kr.

© Copyright 2020 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jul 31, 2020 ; Revised: Aug 12, 2020 ; Accepted: Aug 18, 2020

Published Online: Sep 03, 2020

Abstract

The formation of biofilms on the enamel surface of teeth by Streptococcusmutans is an important step in dental plaque formation, demineralization, and early caries because the biofilm is where other bacteria involved in dental caries attach, grow, and proliferate. The objectives of this study were to determine the effect of phosvitin (PSV) on the biofilm formation, exopolysaccharides (EPS) production, adherence activity of S. mutans, and the expression of genes related to the compounds essential for biofilm formation (quorum-sensing inducers and components of biofilm matrix) by S. mutans. PSV significantly reduced the biofilm-forming activity of S. mutans and increasedthe degradation of preformed biofilms by S. mutans. PSV inhibited the adherence activity of S. mutans by 31.9-33.6%, and the production of EPS by 62-65% depending upon the strains and the amount of PSV added. The expressions of genes regulating the production of EPS and the quorum-sensing-inducers (gtfA, gtfD, ftf, relA, vicR, brpA, and comDE) in all S. mutans strains were down-regulated by PSV, but gtfB was down-regulated only in S. mutans KCTC 5316. Therefore, the anti-biofilm-forming activity of PSV was accomplished through the inhibition of biofilm formation, adherence activity, and the production of quorum-sensing inducers and EPS by S. mutans.

Keywords: phosvitin; Streptococcus mutans; biofilm formation


Journal Title Change

The name of the journal has been changed from Korean Journal for Food Science of Animal Resources to Food Science of Animal Resources from January 2019.

Clarivate Analytics, which manages SCIE, acknowledges that the journal has been renamed, and below is a screenshot of the journal name change in Journal Citation Reports.

Thank you for your interest in our journal.




Download Screenshot
I don't want to open this window for a day.