Anti-biofilm activities of Manuka honey against Escherichia coli O157:H7

Su-Yeon Kim¹, Seok-Seong Kang¹^,^*

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¹Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University, Goyang 10326, Korea.

^*Corresponding Author: Seok-Seong Kang, Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University, Korea. Phone: +82-31-961-5150. E-mail: sskang@dongguk.edu.

© Copyright 2020 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 25, 2020 ; Revised: May 18, 2020 ; Accepted: May 31, 2020

Published Online: Jun 05, 2020

Abstract

Manuka honey (MH) has been shown anti-bacterial activity against several pathogenic bacteria. However, the inhibitory effect of MH on biofilm formation by Escherichia coli O157:H7 has not yet been examined. In this study, MH significantly reduced E. coli O157:H7 biofilm. Moreover, pre- and post-treatment with MH also significantly reduced E. coli O157:H7 biofilm. Cellular metabolic activities exhibited that the viability of E. coli O157:H7 biofilm cells was reduced in the presence of MH. Further, colony forming unit of MH-treated E. coli O157:H7 biofilm was significantly reduced by over 70%. Collectively, this study suggests the potential of anti-biofilm properties of MH which could be applied to control E. coli O157:H7.

Keywords: Manuka honey; Escherichia coli O157:H7; Biofilm; Anti-bacterial