ARTICLE

Muscle Fiber Characteristics on Chop Surface of Pork Loin (M. longissimus thoracis et lumborum) Associated with Muscle Fiber Pennation Angle and Their Relationships with Pork Loin Quality

Sumin Song1https://orcid.org/0000-0001-7115-2253, Huilin Cheng1https://orcid.org/0000-0003-0628-3358, Eun-Young Jung2https://orcid.org/0000-0001-5739-4280, Seon-Tea Joo3https://orcid.org/0000-0002-5483-2828, Gap-Don Kim1,2,*https://orcid.org/0000-0001-5870-8990
Author Information & Copyright
1Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea
2Institutes of Green Bio Science & Technology, Seoul National University, Pyeongchang 25354, Korea
3Division of Applied Life Science (BK21+), Gyeongsang National University, Jinju 52852, Korea
*Corresponding author : Gap-Don Kim, Graduate School of International, Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea, Tel: +82-33-339-5778, Fax: +82-33-339-5779, E-mail: gapdonkim@snu.ac.kr

© Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jun 24, 2020 ; Revised: Aug 01, 2020 ; Accepted: Aug 03, 2020

Published Online: Nov 01, 2020

Abstract

The influence of muscle architecture on muscle fiber characteristics and meat quality has not been fully elucidated. In the present study, muscle fiber characteristics on the chop surface of pork loin (M. longissimus thoracis et lumborum, LTL), pennation angle degree, and meat quality were evaluated to understand the pork LTL architecture and its relationship with the loin chop quality. Muscle fiber pennation degree ranged from 51.33° to 69.00°, resulting in an ellipse-shaped muscle fiber on the surface of pork loin chop. The cross-sectional area (CSA) on the sections cut vertical to the muscle length (M-Vertical) was considerably larger (p<0.05) than that on the sections cut vertical to the muscle fiber orientation (F-Vertical) regardless of the fiber type. Pennation angle is positively correlated with CSAs of F-Vertical (p<0.05) and with Warner-Bratzler shear force (r=0.53, p<0.01). Besides the shear force, lightness and pH were positively correlated with the fiber composition and CSA of IIX fiber (p<0.05); however, the redness, yellowness, drip loss, and cooking loss were not correlated with the pennation angle and muscle fiber characteristics on the chop surface (p>0.05). These observations might help us in better understanding pork loin architecture and the relationship between the pennation angle, muscle fiber characteristics, and meat quality of pork loin chop.

Keywords: muscle architecture; pennation angle; muscle fiber characteristics; pork loin

Introduction

Muscle fiber type distribution, relative composition, fiber size, total number, and fiber density have been considered as the major criteria for assessing meat quality, growth performance, and carcass characteristics of livestock animals (Bee et al., 2007; Chang et al., 2003; Choi and Kim, 2009; Hwang et al., 2010; Joo et al., 2013; Lebret et al., 1999; Lefaucheur et al., 2002; Ozawa et al., 2000; Ryu and Kim, 2006). These muscle fiber characteristics are assessed based on the muscle functions in the body, thereby resulting in different physiological and morphological properties of each muscle (Maclntosh et al., 2006; McDonald et al., 1997). Individual muscles have their own muscle fiber architecture and are classified into several types, such as fusiform, pennate, and parallel muscles (Narici, 1999; Nigg and Herzog, 1994). Among the skeletal muscles, present in the mammalian body, M. longissimus thoracis et lumborum (LTL) is located on the vertebrae and supports and regulates the body movements. Accordingly, the LTL muscle is pennate type and its pennation angle ranges from 31.36° to 83.33° regardless of the species (Derington et al., 2011; Kim et al., 2018). Owing to the diverse muscle fiber architecture, muscle fibers of inconsistent faces are exposed to the muscle surface while cutting meat pieces or chops. In pork loin chops, an oval shape is predicted due to the leaned muscle fibers in the LTL muscle. Moreover, a changeable shape is expected due to the angle degree of muscle fiber pennation while considering the pennation angle ranges from 48.00° to 83.33° in porcine LTL muscle (Kim et al., 2018).

Previous studies on muscle fiber characteristics have mainly focused on their relationships with carcass traits and meat quality (Chang et al., 2003; Choi and Kim, 2009; Hwang et al., 2010; Joo et al., 2013; Kim et al., 2010; Kim et al., 2013a, 2013b; Ozawa et al., 2000; Ryu and Kim, 2006); however, skeletal muscle morphology and muscle fiber architecture have not been fully addressed from the meat science point of view. In addition, loin steak is normally prepared by cutting vertically to the muscle length. Consequently, meat quality characteristics, such as color, water-holding capacity, and tenderness may be affected by the chop surface condition, especially the characteristics of the muscle fibers on the chop surface, as it has been demonstrated in beef striploin influenced by the muscle fiber angle (Derington et al., 2011). Therefore, in the present study, the degree of pennation angle was evaluated to understand the morphological characteristics (muscle fiber architecture) of porcine LTL muscle and its influence on the muscle fiber characteristics of the pork loin chop surface. Furthermore, their relationships with meat quality in pork loin chop was assessed.

Materials and Methods

Sample preparation

Pig carcasses (n=20, crossbred of Landrace×Yorkshire×Duroc, 82.5±3.8 kg of carcass weight) were randomly selected at 24 h postmortem at a commercial slaughterhouse. Whole loin muscle (M. longissimus thoracis et lumborum, LTL) was removed from the left side of each carcass. Loins were cut vertically to a muscle length of 15 cm. Three chops of the medial region of each loin were selected and randomly allocated to three groups: 1) chops prepared by cutting vertical to the length of LTL (M-Vertical), 2) chops prepared by cutting vertical to the muscle fiber orientation (F-Vertical), and 3) chops for measuring the loin-eye area and pennation angle of muscle fiber. For the F-Vertical and M-Vertical groups, each piece of LTL muscle was divided into 3.0-cm-thickness chops. The LTL chop of the F-Vertical group was used to analyze muscle fiber characteristics, whereas that of the M-Vertical group was used to assess muscle fiber characteristics and meat quality.

Loin-eye area and pennation-angle degree

Loin-eye area was measured by tracing the surface of the LTL chop using acetate paper. The tracings were measured using an Image Pro Plus Program (Media Cybernetics, Rockville, MD, USA) after scanning by a high-resolution scanner (11000XL, EPSON, Nagano, Japan). To measure the muscle fiber pennation angle (Fig. 1A), we applied the Kim et al. (2018) method with some modifications. Briefly, the LTL chop was prepared by cutting it 2.0 cm in thickness and again parallel to the muscle length. The muscle fiber lean degree to the LTL muscle fascial membrane was calculated using a protractor.

kosfa-40-6-957-g1
Fig. 1. Schematic representation of pork loin (M. longissimus thoracis et lumborum) architecture and stained cross-sections. (A) Pork loin cut parallel to the muscle length and image of the chop surface cut vertical to the muscle length. Pennation angle (θ), learning degree of muscle fiber to the fascia of loin muscle; arrows with broken line, muscle fiber orientation. (B) Schematic representation of muscle fiber and cross-sections: F-Vertical, cut vertical to the muscle fiber orientation; M-Vertical, cut vertical to the muscle length. CSAF, cross-sectional area of F-Vertical; CSAM, cross-sectional area of M-Vertical. (C) Cross-sections stained with anti-myosin heavy chain (MHC) antibodies. Images were presented after merging four images obtained from different anti-MHC antibodies (BA-F8, SC-71, BF-35, and BF-F3; DSHB, IA, USA). Muscle fiber types were distinguished with different colors (I, pink; IIA, yellowish green; IIX, green; IIB, blue). Bar=100 μm.
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Muscle fiber characteristics

Muscle pieces (1.0×1.0×1.5 cm) were cut from each chop of M-Vertical and F-Vertical groups and immediately frozen in 2-methybutane chilled with liquefied nitrogen. 10-μm-thickness sections were obtained by making parallel cuts to the chop surface of M-Vertical group and by making vertical cuts to the muscle fiber orientation of F-Vertical group, respectively, using a cryostat microtome (CM1520, Leica Biosystem, Wtezlar, Germany). The method by Song et al. (2020) with some modifications was used to stain the cross-sections and muscle fiber typing. Briefly, each section was incubated with monoclonal anti-myosin heavy chain (MHC) antibodies purchased from DSHB (Iowa, IA, USA), such as BA-F8 (anti-MHC slow/I), SC-71 (anti-MHCs 2a and 2x), BF-35 (anti-MHCs slow/I and 2a), and BF-F3 (anti-MHC 2b). Secondary antibodies (anti-mouse IgG2b, anti-mouse IgG1, and anti-mouse IgM) conjugated with fluorescent dyes (Alexa Fluor 350, 488, 594, and 647; Thermo Fisher Scientific, Waltham, MA, USA) were used. Primary and secondary antibodies were applied to the sections for 1 h at room temperature in a dark container. Images were captured using a confocal scanning laser microscope (TCS SP8 STED, Leica Biosystems). Three images obtained from the different regions of each section were analyzed using Image-Pro Plus 5.1 (Media Cybernetic, Rockville, MD, USA). Based on the specificities of anti-MHC antibodies to MHCs, the muscle fibers were classified as I (MHC I/slow), IIA (MHC 2a), IIX (MHC 2x), and IIB (MHC 2b). Hybrid fibers comprising two or more isoforms of MHC were excluded from this study due to their low frequencies. Approximately 500 fibers per sample were typed and their relative fiber compositions (number or area to the total number or area of fibers, respectively, %), cross-sectional area (CSA, μm2), and total number of fibers (TNF) distributed on the loin-eye (chop surface) were analyzed.

Meat quality

The pH of LTL chop was directly measured via a portable pH meter (Seven2Go, Mettler-Toledo, Greifensee, Switzerland), with triplication into different regions. Meat color on the chop surface was assessed after oxygenation of myoglobin via exposure to air for 30 min using a colorimeter (CR-400, Minolta, Tokyo, Japan). Prior to measuring meat color, the colorimeter was calibrated with a white plate (Y=93.5, x=0.3132, y=0.3198). The color values were expressed by a Commission Internationale de l’Eclairage (CIE, 1978) system, such as lightness (CIE L*), redness (CIE a*), and yellowness (CIE b*). Drip loss was analyzed by suspending the LTL chops in a plastic bag for 24 h at 4°C according to the method of Honikel (1987), with certain modifications. After suspension, the LTL chops were weighed and drip loss was presented as a percentage of the initial weight. Cooking loss was measured by cooking the LTL chops in a water bath at 75°C after packing them in a plastic bag. When the internal temperature reached 70°C, the chops were removed from the water bath and cooled to room temperature. Cooking loss was presented as a percentage of the initial weight of the LTL chop. After measuring cooking loss, the chops were used for Warner-Bratzler shear force (WBSF) measurements. Three cores (1.3 cm in diameter) were obtained from each chop by making parallel cuts to the chop surface. The cores were cut vertically using a texture analyzer TA1 (Ametek, Largo, FL, USA) with a V-shaped blade, and the WBSF values (N/cm2) were presented as the average of the three cores.

Statistical analysis

Experimental data obtained from each pork chop are presented as standard error of means (SE). Data was statistically analyzed using SAS 9.4 (SAS Institute, Cary, NC, USA). To compare the relative CSA and fiber area composition between the F-Vertical and M-Vertical groups and among the muscle fiber types, t-tests were performed within the same fiber type or within the same group. Correlation coefficients between pennation angle, loin-eye area, muscle fiber characteristics, and meat quality traits were analyzed using the CORR procedure. Values of p<0.05, p<0.01, and p<0.0001 were considered as statistically significant.

Results

Architecture, muscle fiber characteristics, and meat quality of porcine LTL muscle

Porcine LTL is unipennate type muscle, as illustrated in Fig. 1. Due to the leaned muscle fibers, the surface of the loin chop cut vertically to the length of the muscle had ellipse-shaped muscle fibers regardless of the fiber type. Muscle fiber characteristics (relative number, relative area, CSA, and TNFs) were evaluated using the cross-section prepared by making a vertical cut to the muscle fiber orientation (F-Vertical) as well as by using the section cut vertically to the muscle length (M-Vertical) (Fig. 1B). Their basic data, loin-eye area, and pennation angle degree are listed in Table 1. The loin-eye area was 52.90 cm2. Muscle fibers were leaned at 51.33° to 69.00° from the pennation angle. For the F-Vertical muscle fiber characteristics, the relative number and area of muscle fiber varied according to the fiber type, and relatively higher compositions were found in type IIB (65.97% relative number and 75.10% relative area). Types I and IIA presented numerically lower compositions of both fiber number and area than those observed in type IIX or IIB. The CSA of types IIB and IIX was larger than that of type II or IIA. Moreover, the CSA of F-Vertical (CSAF) was higher in types IIX or IIB than that in type I or IIA. A similar result was observed for fiber composition (relative fiber number and area) in M-Vertical when compared to that in F-Vertical; however, CSA in M-Vertical (CSAM) was larger than that in F-Vertical, with approximately twice the size, regardless of the fiber type (Table 1 and Fig. 2). For meat quality characteristics, a pH range of 5.40–5.62 was observed in pork loin chop. The CIE L*, CIE a*, and CIE b* on the chop surface were 51.06, 5.51, and 5.62, respectively. Drip loss was 2.62% with 0.65% of SD, whereas cooking loss was 22.16% with 2.78% of SD. The WBSF values ranged from 27.58 N/cm2 to 47.34 N/cm2, as listed in Table 1.

Table 1. Basic data (mean, SD, min, and max) of porcine longissimus thoracis et lumborum muscles
Variables Mean SD Min. Max.
Pennation angle (°) 60.44 4.42 51.33 69.00
Loin-eye area (cm2) 52.90 4.93 45.16 58.00
Muscle fiber characteristics
 F-Vertical1)
  Relative fiber number (%) I 10.30 2.73 5.19 17.21
IIA 9.59 3.19 4.87 16.56
IIX 13.72 3.14 9.42 20.45
IIB 65.97 5.32 53.90 75.00
  Relative fiber area (%) I 6.42 1.60 3.99 9.50
IIA 5.46 1.97 2.51 9.43
IIX 12.86 3.58 7.09 20.98
IIB 75.10 4.50 66.83 83.48
  Cross-sectional area (μm2) I 2,976.77 604.90 2,078.12 4,222.43
IIA 2,708.69 523.81 1,952.74 3,810.24
IIX 4,438.99 728.74 3,372.31 5,770.76
IIB 5,489.73 755.18 4,340.15 7,172.77
 M-Vertical2)
  Total number of fiber (×106) 0.60 0.12 0.41 0.83
  Relative fiber number (%) I 11.91 1.65 4.83 16.08
IIA 8.72 1.53 5.11 13.22
IIX 15.48 2.28 11.52 19.81
IIB 63.17 4.37 54.10 72.94
  Relative fiber area (%) I 6.37 1.78 3.12 9.75
IIA 5.53 2.23 2.11 10.18
IIX 13.01 4.35 7.13 24.56
IIB 75.08 5.59 61.54 85.72
  Cross-sectional area (μm2) I 6,310.86 1,631.94 3,781.78 9,300.70
IIA 5,643.51 1,350.22 3,910.83 8,493.19
IIX 9,247.79 2,314.12 6,136.95 15,991.19
IIB 11,427.02 2,248.79 8,050.47 16,079.25
Meat quality
 pH 5.58 0.06 5.40 5.62
 Meat color
  CIE L* 51.06 2.44 46.22 55.84
  CIE a* 5.51 0.80 4.45 7.14
  CIE b* 5.62 1.74 3.26 8.87
 Drip loss (%) 2.62 0.65 1.42 3.54
 Cooking loss (%) 22.16 2.78 16.34 27.94
 Warner-Bratzler shear force (N/cm2) 33.49 5.47 27.58 47.34

1) Cross-sections prepared by cutting vertical to the muscle fiber orientation.

2) Cross-sections prepared by cutting vertical to the muscle length.

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kosfa-40-6-957-g2
Fig. 2. Comparison of muscle fiber size and relative fiber area between the different cross-sectional cuts and muscle fiber types. Relative cross-sectional areas (CSA) (A) and relative fiber area (B) were compared for cross-sections between F-Vertical (cut vertical to the muscle fiber orientation) and M-Vertical (cut vertical to the muscle length) and among the muscle fiber types. Different letters on the bar indicate significant differences between F-Vertical and M-Vertical groups (x, y) within the same fiber type or among different muscle fiber types (a–c) within the same cross-sectional group at p<0.05.
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Relationships between loin-eye area, pennation angle, and muscle fiber characteristics

Table 2 presents the results of correlation coefficients between the loin-eye area, pennation angle, and muscle fiber characteristics. Loin-eye area was correlated only with the relative area of type IIB regardless of the cross-section type (r=0.39, p<0.05 for F-Vertical; r=0.42, p<0.05 for M-Vertical). Pennation angle revealed negative correlations with CSAM of all fiber types (p<0.05), whereas the other traits of muscle fiber characteristics and loin-eye area were not significantly correlated with the pennation angle (p>0.05). TNF was negatively correlated with the pennation angle (r=−0.45, p<0.05). The relative number of type I was positively correlated with TNF (p<0.05), whereas that of type IIB presented negative correlation with TNF (p<0.01) regardless of the cross-section type. Nevertheless, no significant correlation was found in all compositions of the fiber area in F-Vertical as well as M-Vertical (p>0.05). CSAM of all fiber types exhibited negative correlations with TNF (p<0.01), whereas no significant correlations were observed in any type of muscle fiber in F-Vertical between TNF - and CSAF (p>0.05).

Table 2. Correlation coefficients between loin-eye area, pennation angle, and muscle fiber characteristics of pork loin chops
Variables Loin-eye area Pennation angle TNF1)
Pennation angle –0.45*
Loin-eye area 0.19 0.05
F-Vertical2)
 Relative number I –0.29 –0.18 0.49**
IIA –0.03 –0.03 0.07
IIX –0.27 0.17 0.24
IIB 0.07 0.15 –0.56**
 Relative area I –0.18 0.03 0.24
IIA 0.00 –0.23 0.30
IIX –0.21 0.30 0.09
IIB 0.39* 0.04 –0.32
 Cross-sectional area I –0.18 0.03 0.24
IIA 0.00 –0.23 0.31
IIX –0.21 0.30 0.09
IIB 0.32 0.04 –0.32
M-Vertical3)
 Relative number I –0.25 –0.08 0.41*
IIA 0.05 0.02 0.14
IIX –0.16 0.16 0.29
IIB 0.13 0.17 –0.51**
 Relative area I –0.26 0.00 0.31
IIA –0.11 –0.17 0.15
IIX –0.33 0.24 –0.06
IIB 0.42* –0.11 –0.12
 Cross-sectional area I –0.09 0.55** –0.78***
IIA –0.32 0.51* –0.71**
IIX –0.27 0.68** –0.79***
IIB 0.14 0.57** –0.83***

1) Total number of fiber on chop surface of pork loin.

2) Cross-sections prepared by cut vertical to the muscle fiber orientation.

3) Cross-sections prepared by cut vertical to the muscle length.

* p<0.05,

** p<0.01,

*** p<0.0001.

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Relationships between loin-eye area, pennation angle, and muscle fiber characteristics on chop surface and meat quality

Among the meat quality traits, pH, CIE L*, and WBSF were significantly correlated with the pennation angle or muscle fiber characteristics (p<0.05), whereas other meat quality traits such as CIE a*, CIE b*, drip loss, and cooking loss did not indicate significant correlations with the pennation angle or muscle fiber characteristics (p>0.05; Table 3). Moreover, the loin-eye area did not reveal significant correlations with any meat quality traits (p>0.05). pH was negatively correlated with TNF (r=−0.45, p<0.05) but positively correlated with CSAM of type IIX (r=0.36, p<0.05) and IIB (r=0.39, p<0.05) fibers. CIE L* was positively correlated with type IIX in the relative fiber number or relative area (p<0.05). WBSF was positively correlated with the pennation angle (r=0.53, p<0.01), relative fiber area (r=0.55, p<0.01), and CSAM (r=0.41, p<0.05) of type IIX; whereas it was negatively correlated with the relative fiber area of type IIB (r=−0.55, p<0.01).

Table 3. Correlation coefficients between loin-eye area, pennation angle, muscle fiber characteristics on chop surface of pork loin, and meat quality characteristics
Variables pH Meat color Drip loss Cooking loss WBSF
CIE L* CIE a* CIE b*
Pennation angle 0.09 0.16 0.32 0.14 0.18 –0.10 0.53**
Loin-eye area –0.10 0.16 0.19 0.06 –0.22 –0.10 0.07
Total number of fiber –0.45* 0.12 –0.20 0.18 –0.09 0.16 –0.33
Relative fiber number I –0.11 –0.15 –0.06 0.02 –0.06 0.27 –0.02
IIA 0.14 0.12 0.08 0.08 0.19 0.30 0.31
IIX –0.02 0.44* –0.18 0.15 0.09 0.31 0.11
IIB 0.07 –0.14 0.28 0.00 0.07 –0.24 –0.21
Relative fiber area I –0.20 –0.22 –0.05 –0.09 –0.17 0.10 0.00
IIA 0.00 –0.10 0.05 0.08 0.03 0.23 0.29
IIX 0.31 0.35* 0.04 0.10 0.27 0.30 0.55**
IIB –0.18 –0.18 –0.04 –0.08 –0.17 –0.32 –0.55**
Cross-sectional area I 0.30 –0.31 –0.26 –0.31 –0.23 –0.04 0.25
IIA 0.31 –0.12 –0.01 –0.09 0.09 –0.19 0.18
IIX 0.36* –0.08 –0.02 –0.19 0.10 0.02 0.41*
IIB 0.39* –0.18 –0.07 –0.32 –0.26 –0.14 0.24

* p<0.05,

** p<0.01.

WBSF, Warner-Bratzler shear force.

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Discussion

Muscle fibers were leaned at 51.33° to 69.00° in the porcine LTL muscle, which is within the range of previous observations in porcine LTL (48.00° to 83.33°) or bovine longissimus lumborum (31.36° to 53.90°) muscles (Derington et al., 2011; Kim et al., 2018). Pennation angle was not significantly correlated with the loin-eye area but was considerably correlated with the total number and fiber size on the loin-eye (chop) surface (Table 2). As illustrated in Fig. 1B, CSA is dependent on the pennation degree (θ) of muscle fiber, that is, LTL with a smaller θ has smaller CSAM but LTL comprising muscle fiber with bigger θ has larger CSAM regardless of the original CSA (transverse to the muscle fiber orientation) of the fibers. Consequently, TNF on the loin-eye is negatively correlated with both the pennation angle and CSAM of muscle fibers regardless of the fiber type. Furthermore, a negative correlation between TNF and muscle fiber size was previously demonstrated, and these parameters are considered for muscle development and muscle mass, which are influenced by various factors (species, breed, gender, hormones, genotype, and growth promoters) (Rehfeldt and Ender, 1993; Rehfeldt et al., 2000; Ryu et al., 2004; Ullman and Oldfors, 1989). Both are positively correlated with muscle mass, whereas the results observed in this study were not correlated with the loin-eye area. Whether these inconsistent results seem to be a result of the muscle fiber architecture (unipennate type of LTL muscle) and pennation degree remains unclear. Further studies on the relationships between TNF, CSA, and muscle mass in LTL muscle by considering the muscle fiber architecture are warranted to understand the muscle development of livestock animals in detail.

Muscle fibers exposed to the surface of the loin chop look elliptical and their size is considerably larger than that of the transverse section (Fig. 2A); however, muscle fiber composition (relative area) did not differ between the F-Vertical and M-Vertical. The size and composition relationships in glycolytic fibers (IIX and IIB) with pH and tenderness observed in the present study are in accordance with the findings in previous studies that demonstrated their positive or negative correlations with pH or tenderness, respectively, regardless of the animal species, breed, gender, age, and muscle type (Choi and Kim, 2009; Joo et al., 2013; Karlsson et al., 1993; Kim et al., 2013a; Kim et al., 2018; Larzul et al., 1997; Ryu et al., 2008). The CIE L* and CIE a* in meat color are highly affected by the fiber size and composition (Hwang et al., 2010; Kim et al., 2010; Kim et al., 2013a; Ozawa et al., 2000), that is, oxidative fibers are positively correlated with CIE a*, whereas glycolytic fibers are negatively correlated with CIE a* but positively correlated with CIE L* due to their diverse reliance on oxygen and consequent demands of myoglobin for energy metabolism of different muscle fiber types (Cassens and Cooper, 1971; Kim et al., 2010; Ozawa et al., 2000; Whipple et al., 1992). In this study, type IIX, one of the glycolytic fibers, revealed positive correlations in its relative area and CSA with CIE L*; however, except for these results, we did not find any relationship between the muscle fiber characteristics and color traits. These results are observed due to the difference in shape and size of the muscle fiber cross-section between F-Vertical and M-Vertical. F-Vertical is a general type of muscle fiber cross-section, whereas M-Vertical is considerably larger and elliptical in shape, as aforementioned. Moreover, the converse relationships of WBSF were observed in relative fiber areas of type IIX (positive) and IIB (negative). The fiber area composition and size of IIX revealed a similar relationship to tenderness when compared to the previous observations in beef and pork (Karlsson et al., 1993; Renand et al., 2001). The positive correlation between the pennation angle and WBSF indicates that the shear force increased because the muscle fiber cross-section came closer to the longitudinally cut shape as the muscle fibers gradually leaned with the increase in the pennation angle.

Conclusion

Pennation angle degree determines the muscle fiber shape, size, and TNFs on loin eye (chop surface). In particular, muscle fiber size is highly affected by the pennation angle regardless of the fiber type. Consequently, muscle fiber size and composition of glycolytic fibers (IIX and IIB) are closely related to the pH, CIE L*, and tenderness of loin chop. A high degree of pennation angle and large size of IIX fiber negatively affected the tenderness of the loin chop. In conclusion, these observations help to understand the porcine LTL muscle architecture and the influence of muscle fiber characteristics of the chop surface on the meat quality of pork loin.

Conflicts of Interest

The authors declare no potential conflicts of interest.

Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by Korea government (MSIT) (NRF-2019R1C1C1011056).

Author Contributions

Conceptualization: Song SM, Joo ST, Kim GD. Data curation: Song SM, Kim GD. Formal analysis: Song SM, Cheng H, Jung EY. Methodology: Song SM, Cheng H, Jung EY. Writing – original draft: Song SM. Writing – review & editing: Song SM, Cheng H, Jung EY, Joo ST, Kim GD.

Ethics Approval

This article does not require IRB/IACUC approval because there are no human and animal participants.

References

1.

Bee G, Calderini M, Biolley C, Guex G, Herzog W, Lindemann MD. 2007; Changes in the histochemical properties and meat quality traits of porcine muscles during the growing-finishing period as affected by feed restriction, slaughter age, or slaughter weight. J Anim Sci. 85:1030-1045

2.

Cassens RG, Cooper CC. 1971; Red and white muscle. Adv Food Res. 19:1-74

3.

Chang KC, da Costa N, Blackley R, Southwood O, Evans G, Plastow G, Wood JD, Richardson RI. 2003; Relationships of myosin heavy chain fibre types to meat quality traits in traditional and modern pigs. Meat Sci. 64:93-103

4.

Choi YM, Kim BC. 2009; Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality. Livest Sci. 122:105-118

5.

CIE [Commission Internationale de l’Eclairage]. 1978 Reccommendations on uniform color spaces-color difference equations, Psychometric Color Terms. Supplement No. 2 to CIE Publication No. 15 (E-1.3.1.). Commission Internationale de l’Eclairage. Paris, France:

6.

Derington AJ, Brooks JC, Garmyn AJ, Thompson LD, Wester DB, Miller MF. 2011; Relationships of slice shear force and Warner-Bratzler shear force of beef strip loin steaks as related to the tenderness gradient of the strip loin. Meat Sci. 88:203-208

7.

Honikel KO. 1987; How to measure the water-holding capacity of meat? Recommendation of standardized methods. In Evaluation and control of meat quality in pigs. In: Tarrant PV, Eikelenboom G, Monin G, editors.(ed)Martinus Nijhoof. Dordrecht, The Nederland: pp p. 129-142

8.

Hwang YH, Kim GD, Jeong JY, Hur SJ, Joo ST. 2010; The relationship between muscle fiber characteristics and meat quality traits of highly marbled Hanwoo (Korean native cattle) steers. Meat Sci. 86:456-461

9.

Joo ST, Kim GD, Hwang YH, Ryu YC. 2013; Control of fresh meat quality through manipulation of muscle fiber characteristics. Meat Sci. 95:828-836

10.

Karlsson A, Enfält AC, Essén-Gustavsson B, Lundström K, Rydhmer L, Stern S. 1993; Muscle histochemical and biochemical properties in relation to meat quality during selection for increased lean tissue growth rate in pigs. J Anim Sci. 71:930-938

11.

Kim GD, Kim BW, Jeong JY, Hur SJ, Cho IC, Lim HT, Joo ST. 2013a; Relationship of carcass weight to muscle fiber characteristics and pork quality of crossbred (Korean native black pig×Landrace) F2 pigs. Food Bioprocess Technol. 6:522-529

12.

Kim GD, Jeong JY, Hur SJ, Yang HS, Jeon JT, Joo ST. 2010; The relationship between meat color (CIE L* and a*), myoglobin content, and their influence on muscle fiber characteristics and pork quality. Korean J Food Sci Anim Resour. 30:626-633

13.

Kim GD, Overholt MF, Lowell JE, Harsh BN, Klehm BJ, Dilger AC, Boler DD. 2018; Evaluation of muscle fiber characteristics based on muscle fiber volume in porcine longissimus muscle in relation to pork quality. Meat Muscle Biol. 2:362-374

14.

Kim GD, Ryu YC, Jeong JY, Yang HS, Joo ST. 2013b; Relationship between pork quality and characteristics of muscle fibers classified by the distribution of myosin heavy chain isoforms. J Anim Sci. 91:5525-5534

15.

Larzul C, Lefaucheur L, Ecolan P, Gogue J, Talmant A, Sellier P, Le Roy P, Monin G. 1997; Phenotypic and genetic parameters for longissimus muscle fiber characteristics in relation to growth, carcass, and meat quality traits in large white pigs. J Anim Sci. 75:3126-3137

16.

Lebret B, Le Roy P, Monin G, Lefaucheur L, Caritez JC, Talmant A, Elsen JM, Sellier P. 1999; Influence of the three RN genotypes on chemical composition, enzyme activities, and myofiber characteristics of porcine skeletal muscle. J Anim Sci. 77:1482-1489

17.

Lefaucheur L, Ecolan P, Plantard L, Gueguen N. 2002; New insights into muscle fiber types in the pig. J Histochem Cytochem. 50:719-730

18.

Maclntosh BR, Gardiner PF, Mc Comas A. 2006; Muscle architecture and muscle fiber anatomy. In Skeletal muscle form and function. Human Kinetics. Champaign, IL, USA: pp p. 3-21

19.

Mc Donald KS, Wolff MR, Moss RL. 1997; Sarcomere length dependence of the rate of tension redevelopment and submaximal tension in rat and rabbit skinned skeletal muscle fibers. J Physiol. 501:607-621

20.

Narici M. 1999; Human skeletal muscle architecture studied in vivo by non-invasive imaging techniques: functional significance and application. J Electromyogr Kinesiol. 9:97-103

21.

Nigg BM, Herzog W. 1994 Biomechanics of the musculoskeletal system. John Wiley & Sons. Chichester, UK: .

22.

Ozawa S, Mitsuhashi T, Mitsumoto M, Matsumoto S, Itoh N, Itagaki K, Kohno Y, Dohgo Y. 2000; The characteristics of muscle fiber types of longissimus thoracis muscle and their influences on the quantity and quality of meat from Japanese Black steers. Meat Sci. 54:65-70

23.

Rehfeldt C, Ender K. 1993; Skeletal muscle cellularity and histochemistry in response to porcine somatotrophin in finishing pigs. Meat Sci. 34:107-118

24.

Rehfeldt C, Fiedler I, Dietl G, Ender K. 2000; Myogenesis and postnatal skeletal muscle cell growth as influenced by selection. Livest Prod Sci. 66:177-188

25.

Renand G, Picard B, Touraille C, Berge P, Lepetit J. 2001; Relationships between muscle characteristics and meat quality traits of young Charolais bulls. Meat Sci. 59:49-60

26.

Ryu YC, Choi YM, Lee SH, Shin HG, Choe JH, Kim JM, Hong KC, Kim BC. 2008; Comparing the histochemical characteristics and meat quality traits of different pig breeds. Meat Sci. 80:363-369

27.

Ryu YC, Kim BC. 2006; Comparison of histochemical characteristics in various pork groups categorized by postmortem metabolic rate and pork quality. J Anim Sci. 84:894-901

28.

Ryu YC, Rhee MS, Kim BC. 2004; Estimation of correlation coefficients between histological parameters and carcass traits of pig longissimus dorsi muscle. Asian-Australas J Anim Sci. 17:428-433

29.

Song S, Ahn CH, Kim GD. 2020; Muscle fiber typing in bovine and porcine skeletal muscles using immunofluorescence with monoclonal antibodies specific to myosin heavy chain isoforms. Food Sci Anim Resour. 40:132-144

30.

Ullman M, Oldfors A. 1989; Effects of growth hormone on skeletal muscle. I. Studies on normal adult rats. Acta Physiol Scand. 135:531-536

31.

Whipple G, Hunt MC, Klemm RD, Kropf DH, Goodband RD, Schricker BR. 1992; Effects of porcine somatotropin and supplemental lysine on porcine muscle histochemistry. J Muscle Foods. 3:217-227

Journal Title Change

The name of the journal has been changed from Korean Journal for Food Science of Animal Resources to Food Science of Animal Resources from January 2019.

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