Short Communications

Protective effects of Lacticaseibacillus rhamnosus IDCC3201 on motor functions and anxiety levels in a chronic stress mouse model

Jae Gwang Song1,, Daye Mun2,, Bomi Lee1, Minho Song3, Sangnam Oh4, Jun-Mo Kim5, Jungwoo Yang6, Younghoon Kim2,*, Hyung Wook Kim1,*
Author Information & Copyright
1College of Life Sciences, Sejong University, Seoul 05006, Korea
2Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea
3Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea
4Department of Functional Food and Biotechnology, Jeonju University, Jeonju 55069, Korea
5Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
6Ildong Bioscience, Pyeongtaek-si 17957, Korea

† These authors contributed equally to this work.

*Corresponding Author: Younghoon Kim. E-mail:
*Corresponding Author: Hyung Wook Kim. E-mail:

© Copyright 2023 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jul 28, 2023 ; Revised: Sep 01, 2023 ; Accepted: Sep 13, 2023

Published Online: Sep 21, 2023


Growing evidence indicates a crucial role of the gut microbiota in physiological functions. Gut-brain axis imbalance has also been associated with neuropsychiatric and neurodegenerative disorders. Studies have suggested that probiotics regulate the stress response and alleviate mood-related symptoms. In this study, we investigated the effects of the probiotic Lacticaseibacillus rhamnosus IDCC3201 (L3201) on the behavioral response and fecal metabolite content in an unpredictable chronic mild stress (UCMS) mouse model. Our study shows that chronic stress in mice for three weeks resulted in significant changes in behavior, including lower locomotor activity, higher levels of anxiety, and depressive-like symptoms, compared to the control group. Metabolomic analysis demonstrated that disrupted fecal metabolites associated with aminoacyl-tRNA biosynthesis and valine, leucine, and isoleucine biosynthesis by UCMS were restored with the administration of L3201. Oral administration of the L3201 ameliorated the observed changes and improved the behavioral alterations along with fecal metabolites, suggesting that probiotics play a neuroprotective role.

Keywords: Chronic stress; psychobiotics; Lacticaseibacillus rhamnosus; gut-brain axis; metabolome