A comparative study on the adipogenic and myogenic capacity of muscle satellite cells, and meat quality characteristics between Hanwoo and Vietnamese yellow steers
Received: Mar 28, 2023 ; Revised: May 02, 2023 ; Accepted: May 03, 2023
Published Online: May 23, 2023
Myogenesis and adipogenesis are the important processes determining the muscle growth and fat accumulation livestock, which ultimately affecting their meat quality. Hanwoo is a popular breed and its meat has been exported to other countries. Providing the consumers in the beef importing countries with mechanisms related to its meat quality is necessary to promote the commercialization of Hanwoo beef. This study aimed to compare the myogenesis and adipogenesis properties in satellite cells, and meat quality between Hanwoo and Vietnamese yellow cattle(VYC). Same 28-months old Hanwoo (body weight: 728±45kg) and VYC (body weight: 285±36kg) steers (n=10 per breed) were used. Immediately after slaughter, tissue samples were collected from longissimus lumborum(LL) muscles for satellite cells isolation and assays. After 24 h post-mortem, LL muscles from left carcass sides were collected for meat quality analysis. Under the same in vitro culture condition, the proliferation rate was higher in Hanwoo compared to VYC (p<0.05). Fusion index was almost 3 times greater in Hanwoo (42.17%), compared with VYC (14.93%) (p<0.05). The expressions of myogenesis (MYF5, MYOD1, MYOG and MRF4)- and adipogenesis (PPARγ)-regulating genes, and triglyceride content were higher in Hanwoo, compared with VYC (p<0.05). Hanwoo beef had a higher intramuscular fat and total monounsaturated fatty acids contents than VYC beef (p<0.05). Whilst, VYC meat had a higher a*(redness) value and total polyunsaturated fatty acids content (p<0.05). Overall, there was a significant difference in the in vitro culture characteristics and genes expression of satellite cells, and meat quality between the Hanwoo and VYC.