ARTICLE

Hydrolysis Conditions of Porcine Blood Proteins and Antimicrobial Effects of Their Hydrolysates

Sang Keun Jin1,, Jung Seok Choi2,, Dong-Gyun Yim3
Author Information & Copyright
1Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Jinju, Gyeongnam 52725, Republic of Korea.
2Department of Physiology, Maastricht University, Maastricht, 6229 ER, The Netherlands.
3Department of Animal Science, Sangji University, Wonju 26339, Republic of Korea
*Corresponding Author : Dong-Gyun Yim. Department of Animal Science, Sangji University, Wonju 26339, Korea. Tel: +82-33-730-0537, Fax: +82-33-730-0503, E-mail: tousa0994@naver.com

† These authors contributed equally to this work.

© Copyright 2020 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jun 4, 2019 ; Revised: Dec 3, 2019 ; Accepted: Dec 23, 2019

Published Online: Jan 17, 2020

Abstract

In the present study, we determined the degree of hydrolysis of porcine blood plasma proteins, albumin, and globulin hydrolyzed by six proteases (alcalase, neutrase, flavourzyme, protamex, trypsin, and papain) for various reaction times. Moreover, antimicrobial activities of hydrolysates against five pathogenic microorganisms (Bacillus cereus, Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, and Shigella flexneri) were investigated. Alcalase, trypsin, and papain hydrolysates of the three porcine blood proteins showed higher degree of hydrolysis values than hydrolysates produced by the other three proteases. Degree of hydrolysis of the three porcine blood proteins hydrolyzed by the six proteases failed to increase after 2 h of hydrolysis. In antimicrobial tests, hydrolysates (hydrolysis time of 2 h) showed antibacterial activity only against B. cereus. Albumin hydrolysates showed higher antimicrobial activity than globulin and plasma hydrolysates. Albumin hydrolysates obtained with flavourzyme, protamex, and trypsin showed higher antimicrobial activity than those obtained with the other three proteases.

Keywords: Porcine blood; hydrolysate; protease; antimicrobial activity; Bacillus cereus


Journal Title Change

We announce that the title of our journal and related information were changed as below from January, 2019.

 

Before (~2018.12)

After (2019.01~)

Journal Title

Korean Journal for Food Science of Animal Resources

Food Science of Animal Resources

Journal Abbreviation

Korean J. Food Sci. An.

Food Sci. Anim. Resour.

eISSN

2234-246X

2636-0780

pISSN

1225-8563

2636-0772

Journal Homepage

http://www.kosfaj.org

Same

JCR Citation Indexing

SCIE

SCIE


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