ARTICLE

Garlic Inulin as a Fat Replacer in Vegetable Fat Incorporated Low-Fat Chicken Sausages

Gayathree Nidarshika Jayarathna1https://orcid.org/0000-0001-5618-9146, Dinesh Darshaka Jayasena1https://orcid.org/0000-0002-2251-4200, Deshani Chirajeevi Mudannayake1,*https://orcid.org/0000-0001-8572-0696
Author Information & Copyright
1Department of Animal Science, Uva Wellassa University, Badulla 90000, Sri Lanka
*Corresponding author : Deshani Chirajeevi Mudannayake, Department of Animal Science, Uva Wellassa University, Badulla 90000, Sri Lanka, Tel: +94-55-2226580, Fax: +94-55-2226672, E-mail: deshani@uwu.ac.lk

© Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Nov 01, 2021 ; Revised: Jan 26, 2022 ; Accepted: Jan 28, 2022

Published Online: Mar 01, 2022

Abstract

Inulin is a non-digestible carbohydrate and a prebiotic that can also act as a fat replacer in various foods. This study examined the effect of replacing vegetable oil with garlic inulin on the quality traits of chicken sausages. Water-based inulin gels were prepared using garlic inulin or commercial inulin to imitate fats in chicken sausages. Chicken sausages were prepared separately replacing vegetable oil with water-based inulin gels to reach final inulin percentages of 1, 2, and 3 (w/w). The control was prepared using 3% (w/w) vegetable oil with no inulin. The physicochemical properties and thiobarbituric acid reactive substance (TBARS) value of prepared sausages were analyzed over 28-d frozen storage. Sausages with 2% garlic inulin recorded higher flavour and overall acceptability scores (p<0.05). Ash, moisture, and protein contents of the sausages were increased with increasing levels of inulin while fat content was reduced from 13.67% (control) to 4.47%–4.85% (p<0.05) in 3% inulin-incorporated products. Sausages incorporated with 2% inulin had lower lightness (L*) values than the control (p<0.05). Water holding capacity (WHC) was similar (p>0.05) among the samples. During storage L* value, pH, and WHC decreased while redness (a*) and yellowness (b*) values increased in all the samples. In addition, TBARS values were increased during the storage in all samples within the acceptable limits. In conclusion, garlic inulin can be used successfully as a fat substitute in sausages without altering meat quality parameters.

Keywords: fat substitute; garlic; meat quality; prebiotic; thiobarbituric acid reactive substance (TBARS)

Introduction

Meat as a nutritionally dense food of animal origin is vital for a healthy and well balanced diet. Meat and meat products have a unique role in optimal human growth and development throughout the history (Higgs, 2000; Pereira and Vicente, 2013). These are rich in protein of high biological value, in particular essential amino acids such as isoleucine, leucine, lysine, methionine, tryptophan, valine, and phenylalanine, and micro nutrients like vitamin B12, vitamin A, folic acid, iron, selenium, zinc, and copper that human body needs to function properly (Pereira and Vicente, 2013; Verbeke et al., 2010). Despite the nutritional value, meat and meat products are considered disease-promoting food primarily due to the fat content (Pereira and Vicente, 2013) that is originally present and/or added during processing including vegetable oil. Some epidemiological data has revealed that prolonged intake of high fat meat products is directly associated with the occurrence of several chronic diseases such as cancer and cardiovascular diseases (Domingo and Nadal, 2017; Han and Bertram, 2017).

Fat as a vital ingredient in the production of meat products contributes to the flavor, texture, mouth feel, and appearance of the end products (Alaei et al., 2018; Cáceres et al., 2004; Luisa García et al., 2006). Commercial sausage manufacturers in Sri Lanka use vegetable oil as a main fat source in their products. Vegetable oils are composed mainly of triacylglycerols (around 95%) along with some free fatty acids, monoacylglycerols, and diacylglycerols and the remainder comprises of minor components which include phospholipids, free and esterified sterols, triterpene alcohols, tocopherols and tocotrienols, pigments, and hydrocarbons (Oswell et al., 2020). However, consumer demand for healthier meat products with lower fat, cholesterol, nitrates, and calories, and higher health promoting components has increased over the last few years (Wolk, 2017). Numerous extensive approaches have been proposed to reduce fat content of meat products by incorporating health promoting ingredients such as dietary fibers while retaining the sensory and organoleptic properties.

Addition of dietary fibers such as inulin into meat products has become an emerging trend in recent years due to their potential health benefits including inhibiting colon tumor growth, lowering the risk of coronary heart diseases, preventing cholesterol absorption and reducing obesity risk (Hathwar et al., 2012). Inulin is being widely applied in the food industry either individually or combined with other related ingredients as a prebiotic, dietary fiber, low calorie sweetener, fat replacer, gelling agent, viscosity modifier, and a texture modifying ingredient in various foods (Melilli et al., 2021; Mensink et al., 2015). In aqueous systems, upon vigorous mixing and consequent cooling, inulin forms a smooth gelling structure which provides a combination of creamier and juicier mouth feel to the end product which can potentially replace fat, without altering its rheological properties but resembling the mouth feel of fat in low-fat meat products upon incorporation (Franck, 2002; Mudannayake et al., 2015b). Hence, it can possibly be used as a fat substitute in formulation of low fat meat products (Illippangama et al., 2022) with a low calorie content of about 1–1.5 kcal per gram (Weiss et al., 2010).

Inulin naturally occurs in tubers, rhizomes, and bulbs of plants such as chicory (Cichorium intybus), garlic (Allium sativum), Jerusalem artichoke (Helianthus tuberosus), leeks (Allium porrum), dahlia (Dahlia pinnata), onion (Allium cepa), asparagus (Asparagus falcaus), etc (Mudannayake et al., 2015a). The commercial production of inulin is primarily based on two plant species, C. intybus and H. tuberosus at present and limited to European countries (Moongngarm et al., 2011). The use of inulin as an ingredient in food industry is very limited in Sri Lanka due to its high cost. Garlic belongs to the family of Amaryllidaceae and Genus Allium. Garlic (A. sativum) bulb is found as a rich source of inulin (18–19 g/100 g fresh weigh; Mudannayake et al., 2015a) and is available in Sri Lanka. Garlic can be used to extract inulin which potentially delivers enhanced flavors and organoleptic properties in food products.

Vegetable oil has been successfully replaced with inulin in the formulation of emulsion-type chicken sausages (Alaei et al., 2018; Berizi et al., 2017). Guedes-Oliveira et al. (2019) produced reduced fat lamb patties incorporating vegetable fat with inulin. Furthermore, Menegas et al. (2013) produced dry-fermented chicken sausage replacing corn oil with inulin for the enhancement of physicochemical, microbiological, and textural characteristics. However, literature reveals that scientific data on effect of garlic inulin as a fat substitute on quality of meat and meat products are scant. Therefore, this study examined the extraction of inulin from garlic and the effects of adding extracted garlic inulin and commercial (chicory) inulin as partial fat replacers on physicochemical, sensory, and textural characteristics of vegetable fat incorporated chicken sausages.

Materials and Methods

Commercial chicory inulin (BENEO Orafti®) was purchased from DPO Lanka (Pvt) Ltd, Colombo, Sri Lanka and garlic inulin was extracted from garlic bulbs using hot water extraction, vacuum evaporation and subsequent spray drying as described by Mudannayake et al. (2015b) with slight modifications. The ingredients employed to prepare the sausage formulations, such as chicken breast meat, vegetable oil, wheat flour, chili powder, salt, pepper, onion, cardamom and cinnamon were purchased from a local grocery store.

Extraction of inulin from garlic

Garlic bulbs (A. sativum) were first peeled, cleaned and washed thoroughly with water and subsequently cut into small slices to have 3 mm thickness. Sliced garlic was then blanched in boiling water, acidified with ascorbic acid (0.1% w/w) for 1 to 2 min, mixed with potable water (1:10 g/mL) and kept at 80±5°C for 50 min with frequent stirring in a double-water-jacketed pan on a stove. Then the mixture was filtered through 4 layers of muslin cloth and the residue was weighed and re-extracted one more time following the same procedure. Both extracts were combined together and concentrated to a brix value of approximately 8° at 60°C using a rotavapor (Model: 1000282711, BUCHI Labortechnik AG, Shanghai, China) equipped with evaporator flask, receiving flask, recirculating chiller, and vacuum pump. The concentrated extracts were finally spray dried (Model: 005-1116-FD, SOLTEC®, Murcia, Spain) at a feed rate of 2.5 L/h, air flow of 110 kg/h, and inlet and outlet temperatures of 60°C and 200°C to obtain a light colour fine powder, respectively.

Fourier transform infrared spectroscopy (FT-IR)

The chemical characterization of extracted garlic inulin and commercially available chicory inulin powders was conducted separately using Fourier-transform infrared (FT-IR) (Bruker FT-IR spectrophotometer, Bruker Optik GmbH, Berlin, Germany) equipped with an OPUS 7.5 FT-IR software against a KBr background. Approximately 2 mg of each powder were milled separately with 200 mg of KBr using a clean motor and pestle and pressed into pellets manually. The prepared clear KBr-inulin pellets were analyzed in the FT-IR. A spectral range of 4,000–400 cm–1 at a resolution of 4 cm–1 and 32 scans for each spectrum were used (Grube et al., 2002).

Determination of inulin content

Inulin content of commercial inulin and extracted garlic inulin powders were analyzed separately according to enzymatic spectrophotometric method (AOAC 999.03) as described by AOAC (2005) using the Megazyme fructan assay kit (Megazyme, Bray, Ireland), which contained sucrase, fructanase, fuctan control, sucrose control and D-Fructose standard (Mudannayake et al., 2015a). Briefly, 1 g of inulin sample was put into a conical flask filled with 80 mL of hot distilled water (~70°C). The conical flasks were covered with parafilm and placed on a shaking water bath at a constant temperature (85±2°C) to extract fructans and sugars. Sample extract was allowed to cool to room temperature and the volume was adjusted to 100 mL with distilled water and it was mixed well. Finally, the mixture was filtered through a filter paper (No. 4 Whatman International, Maidstone, UK) and the inulin content of the extract was determined as described by McCleary et al. (2000). The inulin content of each powder was calculated using the following formula:

Inulin% = Δ A × F × V W × 2.48

where ΔA=sample absorbance-sample blank absorbance (both read against the reagent blank),

F=factor to convert absorbance values to μg of fructose [(54.5 μg of D-fructose) / (absorbance for 54.5 μg of D-fructose)],

V=volume (mL) of extract used, and

W=weight (mg) of sample extracted.

Preparation of water based inulin gel

The water based inulin gel was prepared using 40% of inulin (w/w), 58% of water (w/w) and 2% of soya lecithin (w/w) (Kim and Wang, 2001). During the preparation of inulin gel, the aqueous solution of inulin was heated to 85°C with continuous mechanical stirring for 20 min, mixed with soy lecithin, allowed to cool to room temperature and refrigerated (5°C) for 24 h (Mudannayake et al., 2015a). After 24 h, the resultant water based stable inulin gel was incorporated into meat batter to reach 1%, 2%, and 3% (w/w) total inulin levels in the product.

Experiment-1

Experiment-1 was conducted to select the best level of inulin for manufacture of low-fat chicken sausages without altering the desired quality characteristics.

Formulations

Seven formulations of chicken sausages were prepared for the study with different percentages of laboratory prepared garlic inulin or commercial chicory inulin according to commercial guidelines to test the replacement of vegetable oil with garlic or commercial chicory inulin. The treatments and amounts of the ingredients used in each treatment in production of low-fat chicken sausages are given in Table 1. Chicken meat was first minced in a mincer (HR-2726, Philips, Shanghai, China) and the remaining ingredients (Table 1) were added and mixed thoroughly. The meat batter was then stuffed in cellulose casing and links were formed. The products were then cooked until the core temperature of the product reached to 72°C. After heat treatment, the products were cooled and casings were removed. Finally, the products were vacuum packed (DZ500-2D, Zhejiang Dingye Machinery, Zhejiang, China) and stored under frozen condition (–18°C) until further analysis. The experimental units were 1.5 kg, in triplicate, and at three different time periods (three lots).

Table 1. Percentage ingredients used for the production of low-fat chicken sausages
Ingredients Formulations (% of ingredients)
Control GI 1% GI 2% GI 3% CI 1% CI 2% CI 3%
Chicken breast meat 71 71 71 71 71 71 71
Vegetable oil 3 2 1 0 2 1 0
Garlic inulin gel 0 1 2 3 0 0 0
Commercial inulin gel 0 0 0 0 1 2 3
Wheat flour 6 6 6 6 6 6 6
Ice flakes 15 15 15 15 15 15 15
Chili powder 1.5 1.5 1.5 1.5 1.5 1.5 1.5
Salt 1.5 1.5 1.5 1.5 1.5 1.5 1.5
Pepper 0.5 0.5 0.5 0.5 0.5 0.5 0.5
Onion 1.2 1.2 1.2 1.2 1.2 1.2 1.2
Cardamom 0.15 0.15 0.15 0.15 0.15 0.15 0.15
Cinnamon 0.15 0.15 0.15 0.15 0.15 0.15 0.15

GI, garlic inulin; CI, commercial inulin.

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Proximate composition

Proximate composition of inulin powders and sausages samples was determined following the method of AOAC (2016). Briefly, moisture content (AOAC 950.46) was measured by drying each sample (3 g) in an aluminum dish at 104°C for 15 h (DHG-9145A, Zenith Lab., Changzhou, China). Crude protein content (AOAC 928.08) was measured using the Kjeldahl method (DNP-3000, RAYPA, Barcelona, Spain). Crude protein content was calculated by multiplying the amount of nitrogen by a factor of 6.25. Crude fat content (AOAC 960.39) was measured using the Soxhlet extraction system (EME60250/CEB, Electrothermal, Staffordshire, UK). Crude ash content (AOAC 923.03) was determined by igniting each sample (2 g) in muffle furnace (HD-230, Hobersal, Barcelona, Spain) at 600°C overnight.

Sensory evaluation

The design of the sensory evaluation for cooked sausages was reviewed and approved by the Research Ethics Review Committee of Uva Wellassa University (No. UWU/REC/2021/003). Sensory evaluation of cooked sausages was performed with a panel of 30 untrained panelists in individual booths as described by Menegas et al. (2013). Sausage samples were first heated using a sausage griller (SG 9, ASAIN, Zhengzhou Ohfu Ind. Ent., Zhengzhou, China) just before the evaluation and prepared to uniform size (1.5 cm×2 cm). Coded sausage samples were then evaluated for appearance, texture, odour, flavour, fatty flavour, colour and overall acceptability using a 9-point hedonic scale (9-like extremely, 8-like very much, 7-like moderately, 6-like slightly, 5-neither like nor dislike, 4-dislike slightly, 3-dislike moderately, 2-dislike very much, 1-dislike extremely). Drinking water at room temperature was provided to rinse the mouth prior to and between sample evaluations.

Experiment-2

The best formulations from Experiment-1 were selected based on the results of sensory evaluation for determination of meat quality parameters during a 28-d frozen storage. Prior to analysis, samples were thawed by packing them in a clean leak-proof package and submerging in a container of clean tap water at room temperature, changing the water every 30 min.

Determination of pH value

Each meat sample (1 g) was homogenized with 9 mL of distilled water for 60 s by using a homogenizer (Ika Laboratory Equipment, Seoul, Korea) and filtered through a filter paper (No. 4 Whatman International). The pH value of each filtrate was determined with a pH meter (pH 700, Eutech instruments, Ayer Rajah Crescent, Singapore) after calibration using buffers (pH 4.01, 7.00, and 10.01) at room temperature.

Determination of water holding capacity (WHC)

WHC was determined based on the technique of Hamm (1961), as described by Wilhelm et al. (2010). Sausage samples (2.00±0.10 g) were carefully placed between two pieces of filter papers (No. 4; Whatman International) on acrylic plates and left under a 10-kg weight for 5 min separately. After recording the final weight of each sample, WHC was calculated using the following equation, where Wi and Wf are the initial and final weights (g) of sample, respectively.

WHC ( % ) = 100 [ ( W i W f ) × 100 W i ]
Determination of colour values

The colour values of minced chicken sausages were measured using a colorimeter (CR-410, Konica Minolta, NIC., Japan) which was calibrated against a white reference tile (No. 20433045, Y=85.7, x=0.3170, and y=0.3242, Konica Minolta, NIC., Tokyo, Japan). The average of three repeated measurements in each minced sample was used for the determination of the values of lightness (CIE L*), redness (CIE a*), and yellowness (CIE b*).

Determination of lipid oxidation

Lipid oxidation of sausage samples were evaluated by the determination of 2-thiobarbituric acid relative substances (TBARS) values as described in Lee et al. (2021) with some modifications. Each sample (5 g) was homogenized in 15 mL of deionized water using a homogenizer (D-500, Velp Scientifica, Usmate, Italy) at 14,000 rpm for 30 s. Butylated hydroxytoluene (BHT; 50-μL; 7.2% w/v in ethanol) and thiobarbituric acid/trichloroacetic acid solution (20 mM TBA and 15% [w/v] TCA; 2 mL) were added to the homogenate (1 mL) and vortexed for 30 s. The mixture was then incubated in a water bath (YCW-010E, Gemmy Industrial, Taipei, Taiwan) at 90°C for 30 min, and subsequently cooled for 10 min in an ice-water bath. After centrifuging the samples at 1,409×g for 15 min (5°C) using a ST 40R centrifuge (Thermo Fisher Scientific, Osterode, Germany), the absorbance of was measured at 532 nm with a UV-2005 spectrophotometer (J.P. Selecta, Barcelona, Spain) against a blank prepared with 1 mL deionized water and 2 mL TBA/TCA solution. The malondialdehyde (MDA) concentration of each sample was determined against an external standard curve constructed using tetraethoxypropane. The results were expressed as mg MDA per kg of sausages.

Statistical analysis

The complete experiment was repeated three times in a completely randomized design and duplicate samples were drawn for each parameter. The data were subjected to one-way analysis of variance (ANOVA) and Tukey's comparison of the means test (p<0.05) using Minitab 17 software. Data obtained from sensory analysis was analyzed using the Friedman test.

Results and Discussion

Fourier transform infrared spectroscopy (FT-IR) analysis

Fig. 1 shows the FT-IR spectra of developed garlic inulin and commercial chicory inulin powders. Very identical FT-IR spectra were observed for laboratory prepared garlic inulin powder and commercial inulin powder. Previous studies reported that inulin had distinctive hydroxyl (OH) groups at 3,353 cm–1 (Wu and Lee, 2000) and at 3,384–3,386 cm–1 (Mudannayake et al., 2015b), and C=O at 1,745 cm–1 (Wu and Lee, 2000) and 1,631–1,640 cm–1 (Mudannayake et al., 2015b) in FT-IR analysis. In the present study, the FT-IR spectrum of garlic inulin had absorption bands at 3,339, 1,457, 1,130, and 970 cm–1. The peaks at 970 and 1,130 cm–1 indicated the presence of inulin (Grube et al., 2002). The absorption bands in the range of 3,417–2,924 cm–1 showed the OH groups which are abundant in inulin structure while those in the range of 1,635–1,427 cm–1 are responsible for the presence of esterified carboxyl groups. These confirmed similar chemical structure in inulin from both sources. Furthermore, FT-IR results of the current study are comparable to those of chicory, Jerusalem artichoke, globe artichoke and garlic inulin (Abou-Arab et al., 2011; López-Molina et al., 2005; Wu and Lee, 2000).

kosfa-42-2-295-g1
Fig. 1. Fourier transform infrared (FTIR) spectra of garlic inulin and commercial inulin.
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Inulin content in garlic and commercial inulin powders

Commercial chicory inulin powder contained a higher content of inulin (73.43% dry wt) compared to laboratory prepared garlic inulin powder (63.28% dry wt; Table 2) as measured by enzymatic spectrophotometric method (AOAC 999.03). However, the reported amount of inulin (90% dry wt) in commercial chicory inulin powder as per the product sheet of Orafti (2007) is comparatively higher than the actual quantity of inulin measured enzymatically (73.43% dry wt) in this study. Mudannayake et al. (2015b) attributed such variations between the commercially reported values and the actual measured values to differences in sample preparation and methods of analysis. In addition, a higher level of purity in garlic inulin may be achieved through application of further purification steps such as activated carbon treatment for extracted garlic juice.

Table 2. Chemical constituent composition (%) of garlic and commercial inulin powders
Constituents (%) Garlic inulin powder Commercial inulin powder SEM1)
Moisture 5.10A 3.62B 0.369
Ash 0.94A 0.68B 0.059
Crude protein 0.70B 1.31A 0.154
Crude fat 0.55B 0.67A 0.029
Inulin 63.28B 73.43A 2.36

1) Pooled SEM (n=10).

A,B Values in the same row with different superscripts differ significantly (p<0.05).

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Experiment-1
Proximate composition of inulin powders

The moisture, protein, fat and ash contents of the developed garlic inulin and commercial chicory inulin powders are presented in Table 2. Laboratory prepared garlic inulin powder had lower levels of crude fat and crude protein and higher levels of moisture and ash opposed to commercial inulin powder (p<0.05). The values reported for commercial inulin in this study are not in agreement with those observed by Mudannayake et al. (2015b) in commercial chicory inulin powder. However, Bouaziz et al. (2014) detected almost similar protein content (1.58%) and a higher of ash content (1.19%) in commercial chicory inulin compared to the present study. The differences in the chemical composition might be due to the variations in the species plant extraction process, purification process and method of analysis. In addition, these results suggested that the quality of garlic inulin powder needs to be improved through purification of garlic inulin extract using activated carbon and ion exchange resin (IER) treatments (Mudannayake et al., 2015b).

Proximate composition of chicken sausages

Table 3 shows the proximate composition of chicken sausages as affected by varying levels of garlic and commercial chicory inulin powders. Fat, protein, ash and moisture contents in the sausage samples varied significantly with the incorporation of inulin powder into sausage formulations as a fat replacer, irrespective of the source of inulin.

Table 3. Proximate composition of chicken sausages as affected by different sources and levels of inulin
Constituents (%) Formulations SEM1)
Control GI 1% GI 2% GI 3% CI 1% CI 2% CI 3%
Fat 13.67A 12.89AB 9.47C 4.85D 11.97B 8.12C 4.47D 0.779
Moisture 63.33D 66.11CD 67.10BC 69.74B 69.20BC 73.59A 74.30A 0.846
Ash 1.01C 1.12C 1.17BC 1.20BC 1.19BC 1.38B 2.24A 0.087
Protein 9.65C 9.97BC 10.24ABC 10.76A 10.09ABC 10.24ABC 10.44AB 0.086

1) Pooled SEM (n=21).

A–D Values in the same row with different superscripts differ significantly (p<0.05).

GI, garlic inulin; CI, commercial inulin.

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Sausages with added inulin had lower fat contents than did the control sausages with no added inulin (p<0.05). Replacement of vegetable oil with inulin was the main reason for the observed low fat content in inulin incorporated sausages (Menegas et al., 2013). Additionally, with the increasing levels of inulin in formulations a significant decline in the fat content of sausages was observed, irrespective of the source of inulin. The use of inulin at 0.87%–30% in different types of sausages effectively reduced the fat content of products (Berizi et al., 2017; Bis-Souza et al., 2018; Choi et al., 2016; Glisic et al., 2019; Huang et al., 2011; Keenan et al., 2014; Méndez-Zamora et al., 2015; Menegas et al., 2013; Özer, 2019; Prapasuwannakul, 2018).

According to Table 3, an increase in moisture content was observed in sausages when the oil content was gradually replaced with inulin, irrespective of the source of inulin (p<0.05). However, the effect is more prominent with commercial chicory inulin. The increment in moisture content may be attributed to the capability of inulin to hold more water during preparation and cooking process (El Zeny et al., 2019) due to the presence of hydrophilic groups and the hygroscopic nature of inulin (Alaei et al., 2018). The findings of the present study are in agreement with those reported by several other researchers on effect of substituting fat with inulin on moisture levels in sausages (Berizi et al., 2017; Glisic et al., 2019; Menegas et al., 2013; Özer, 2019). Furthermore, an inverse relationship between fat and moisture contents of sausages formulated with and without inulin was previously observed (de Souza Paglarini et al., 2021; Keenan et al., 2014; Méndez-Zamora et al., 2015; Šojić et al., 2011).

The increased level of substituting vegetable oil with inulin caused an enhancement of protein content in the sausages, irrespective of the inulin source (p<0.05; Table 3). However, the effect was more prominent at an inclusion level of 3% of inulin in sausage formulations. Similarly, studies conducted to evaluate the effect of inulin on quality parameters of different meat products revealed that inulin incorporated samples had higher protein contents than the control samples with no added inulin (Glisic et al., 2019; Méndez-Zamora et al., 2015; Özer, 2019). The increase in protein content in sausages may be due to the substitution of oil with inulin which contained protein levels of 0.70%–1.31%.

Table 3 shows that the inclusion of garlic inulin in to chicken sausages up to 3% had no effect on ash content of chicken sausages as compared with the control sausages (p>0.05). However, the ash contents were significantly higher in sausages with 2% and 3% commercial inulin as opposed to control samples and garlic inulin added sausages (p<0.05). Šojić et al. (2011) and Huang et al. (2011) stated that that inulin added sausages contained higher ash percentages than samples with no added inulin. This is attributed to the higher ash content of inulin powders which is arising from the plant sources.

Sensory analysis

Sensory quality attributes including flavour, colour, and odour determine the consumer preference for food products. Sensory scores given to different sensory attributes of chicken sausages prepared substituting oil with inulin is presented in Fig. 2. The results indicate that substituting the oil content with inulin did not affect the colour and juiciness of sausages (p>0.05). However, significant differences were observed between treatments in relation to texture, odour, flavour, fatty flavour and overall acceptability (p<0.05). Hence, based on the sum of ranks of sensory parameters (Data not shown) substitution of vegetable oil with inulin at 2% was the best level to produce chicken sausage with better acceptability score, irrespective of the inulin source. The increased level of inulin as a fat substitute in chicken sausages enhanced the sensory scores for colour, appearance and texture (Alaei et al., 2018). In contrast, several studies have revealed that inulin did not affect the acceptability of cooked sausages (Cáceres et al., 2004; Luisa García et al., 2006), Chinese-style sausages (Huang et al., 2011) and dry-fermented chicken sausages (Menegas et al., 2013).

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Fig. 2. Radar chart for sensory evaluation to select the best sausages samples.
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Thus, based on the findings of the Experiment-1 it was possible to manufacture chicken sausages with 30.7% and 40.6% lower fat contents with higher product acceptability when vegetable oil was substituted with garlic inulin and commercial inulin at 2% level, respectively. Therefore, these two formulations were selected with the control for analysis of meat quality parameters during frozen storage.

Experiment-2
Change in pH value under frozen storage

Table 4 represents the pH values of chicken sausages as influenced by different formulations and storage period. There was a significant difference in pH values between the control and inulin incorporated sausages (p<0.05) until 21 d of storage. Sausages added with garlic inulin (2%) showed higher pH values as opposed to control sausages and those added with commercial inulin (2%) and this can be attributed to the slightly alkaline nature of garlic. In contrast, addition of inulin and the reduction of fat content had no effect (p>0.05) on the pH of sausages (Mendoza et al., 2001; Menegas et al., 2013; Muguerza et al., 2002) and minced meat (Rodriguez Furlán et al., 2014).

Table 4. Effect of different inulin sources on the pH value of low-fat chicken sausages during storage
Period Formulations SEM1)
Control Garlic inulin Commercial inulin
Day 1 6.18Ay 6.36Ax 6.16Ay 0.032
Day 7 6.14Ay 6.34Ax 6.05Az 0.043
Day 14 6.12Ay 6.24ABx 6.04Az 0.029
Day 21 6.10ABx 6.12BCx 6.02Ay 0.016
Day 28 5.96Bx 6.10Cx 6.00Ax 0.045
SEM2) 0.023 0.030 0.022

1) Pooled SEM (n=9).

2) Pooled SEM (n=15).

A–C Values in the same column with different superscripts differ significantly (p<0.05).

x–z Values in the same row with different superscripts differ significantly (p<0.05).

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The pH values of sausages decreased over the 28-d frozen storage period, particularly in control samples and garlic inulin added samples after 21 d (p<0.05; Table 4). This was probably due to the metabolic activities of psychotropic bacteria and lactic acid bacteria that are considered as the predominant microorganisms in meat products (Borch et al., 1996). Furthermore, ingredients utilized in the formulation (Kim et al., 2011) and interactions between the polysaccharides and polar and non-polar groups of proteins in food via electrostatic association (Choi et al., 2010) may cause for pH reduction. In general, pH value of chicken sausages ranged from 6.5 to 5.0 during a 28-d storage (Kotula and Wang, 1994). The pH values reported in this study were within this range during the frozen storage.

Changes in water holding capacity under frozen storage

WHC of comminuted meats such as sausages is an important quality attribute parameter particularly because the structure of tissues has already been destroyed during the processing of such products (Lawrie and Ledward, 2006). Table 5 shows the WHC of experimental sausages during frozen storage period. Substitution of vegetable oil with inulin had no effect on the WHC of chicken sausages. In contrast, Méndez-Zamora et al. (2015) found that inulin as a fat replacer at 15% and 30% decreased the WHC of low-fat frankfurter compared to the control. However, the same quality attribute was decreased with the increasing storage period in all experimental sausages. This can be attributed to the decrease in pH values observed during the frozen storage as lower pH denatures myofibrils and decrease the WHC (den Hertog-Meischke et al., 1997).

Table 5. Effect of different inulin sources on the WHC (%) of low-fat chicken sausages during storage
Period Formulations SEM1)
Control Garlic inulin Commercial inulin
Day 1 91.83Ax 91.64Ax 90.42Ax 0.624
Day 7 87.30ABx 86.18Bx 86.50Bx 0.538
Day 14 85.43BCx 83.57Bx 83.50BCx 0.451
Day 21 81.04CDx 78.80Cx 80.96Cx 0.555
Day 28 78.14Dx 76.04Cx 77.24Dx 0.636
SEM2) 1.371 1.494 1.245

1) Pooled SEM (n=9).

2) Pooled SEM (n=15).

A–C Values in the same column with different superscripts differ significantly (p<0.05).

x–z Values in the same row with different superscripts differ significantly (p<0.05).

Download Excel Table
Changes in colour values under frozen storage

Changes in colour values of sausages were weekly determined for 28 d under frozen storage (Table 6). All three colour values of sausages were significantly affected by the frozen storage period (p<0.05). In this regards, the lightness (CIE L*) value of control sausages and those enriched with garlic inulin decreased throughout the frozen storage (p<0.05). In contrast, the redness (CIE a*) and yellowness (CIE b*) values increased significantly with increasing storage period, irrespective of the source of inulin. Menegas et al. (2013) reported that sausages became darker during storage; CIE a* value increased (p<0.05) meanwhile CIE L* and CIE b* remained unchanged (p>0.05) during refrigerated storage.

Table 6. Effect of different inulin sources on the CIE colour values of low-fat chicken sausages during storage
Period Formulations SEM1)
Control Garlic inulin Commercial inulin
CIE L*
 Day 1 68.87Ax 65.75Ay 65.97Ay 0.513
 Day 7 68.30Ax 65.41Ay 65.86Az 0.450
 Day 14 65.98Bx 63.26By 65.74Ax 0.450
 Day 21 65.49Bx 63.17By 65.50Ax 0.404
 Day 28 64.73Cy 63.18Bz 65.21Ax 0.308
 SEM2) 0.436 0.315 0.121
CIE a*
 Day 1 11.51Cy 12.07Cx 11.73Cxy 0.104
 Day 7 12.61Bx 12.71Bx 12.73Bx 0.028
 Day 14 14.09Ax 13.55Ay 12.88Bz 0.181
 Day 21 14.24Ax 13.60Ay 13.67Ay 0.108
 Day 28 14.26Ax 13.67Ay 13.86Axy 0.099
 SEM2) 0.297 0.172 0.206
CIE b*
 Day 1 32.71Cx 32.75Cx 33.31Cx 0.140
 Day 7 33.83By 33.46By 34.34Bx 0.137
 Day 14 36.45Ax 34.83Ay 34.58By 0.299
 Day 21 37.13Ax 34.85Ay 36.93Ax 0.382
 Day 28 37.35Ax 34.91Ay 37.27Ax 0.413
 SEM2) 0.507 0.245 0.419

1) Pooled SEM (n=9).

2) Pooled SEM (n=15).

A–C Values in the same column with different superscripts differ significantly (p<0.05).

x–z Values in the same row with different superscripts differ significantly (p<0.05).

Download Excel Table

With respect to the effect of inulin enrichment in sausages on color values, sausages incorporated with garlic inulin and commercial inulin had lower CIE L* values than control sausages until d 7 (p<0.05). Further, the same trend was detected in sausages enriched with garlic inulin as opposed to control until d 28 (p<0.05). The reduced lightness values in inulin-added low-fat sausages can be attributed to the decrease in the shine that is usually provided by fat (Cáceres et al., 2004; Menegas et al., 2013; Nowak et al., 2007). Cáceres et al. (2004) further revealed that the whitish translucent gel formed by inulin has no dominant color. The findings of the current study are similar to those of the previous studies by Berizi et al. (2017) and Öztürk and Serdaroğlu (2017) who observed lower lightness values in inulin-enriched meat products, emulsion type sausages and chicken meat balls, as opposed to those without enrichment, respectively. In contrast, inulin addition significantly increased the CIE L* values in Chinese style sausages compared to control (Huang et al., 2011).

Šojić et al. (2011) found correlations between the CIE L* value and fat content (r=0.99; p<0.001) and also between the CIE a* value and fat content (r=–1.00; p<0.001). Furthermore, Menegas et al. (2013) noticed a higher redness value in sausage formulation with inulin compared with that with standard oil content. Therefore, an increase in redness value was expected with the decrease in the fat level of sausages. However, significantly higher redness values in inulin-added reduced-fat sausages as opposed to control sausages were only observed in d 1. From d 14 onward control sausages had higher redness values than inulin-added sausages (p<0.05). Recently, de Souza Paglarini et al. (2021) observed lower redness values in inulin gel-incorporated Bologna sausages as opposed to control sausages.

Addition of inulin into sausage formulation had no effect on the yellowness of the product at d 1 (p>0.05). From d 7 onward CIE b* value was significantly influenced by the level and source of inulin. In general, sausages added with commercial chicory inulin recorded higher yellowness values than those added with garlic inulin (p<0.05). Comparable yellowness values were reported previously in meat products prepared with and without inulin (Berizi et al., 2017; Prapasuwannakul, 2018).

Textural properties of sausages samples

Texture profile analysis revealed that incorporation of inulin into sausage formulations significantly reduced the hardness (maximum force required to compress the sample) and chewiness (work to masticate the sample for swallowing; p<0.05). Control sample showed a significantly higher hardness value (1,603.7 g) compared with sausages incorporated with commercial inulin (1,165.0 g) and garlic inulin (1,237.0 g). Chewiness value for control sample (47.3 mJ) was significantly greater than that of sausages incorporated with commercial inulin (32.4 mJ) and garlic inulin (39.0 mJ). These results are comparable to those obtained by other authors for frankfurters, pork bologna and beef burger incorporated with dietary fiber (Grigelmo-Miguel et al., 1999; Mansour and Khalil, 1997; Shand, 2000). Reduced-fat meat products incorporated with inulin were softer than those having conventional levels of fat (Cáceres et al., 2004; Choi et al., 2016; Glisic et al., 2019; Özer, 2019; Prapasuwannakul, 2018) which may reduce the amount of force required to chew samples for swallowing.

However, inulin enrichment did not change other texture parameters of sausages such as springiness, cohesiveness, adhesiveness and gumminess (p>0.05; Data not shown). Similar results were reported by Choi et al. (2016) for restructured sausages incorporated with chicory fiber as a fat replacer. Luisa García et al. (2006) reported that differences observed in the texture properties of meat products depend on the type and level of inulin used. When inulin was incorporated as a gel in meat products, textural parameters changed only at a higher level of incorporation (7.5% or more) resulting softer sausages irrespective of the fat content. Therefore, the addition of lower level of inulin in this study (2%) might be the reason for comparable values observed for gumminess, cohesiveness, adhesiveness, and springiness of different sausage samples.

Changes of lipid oxidation with the storage time

To investigate the effect of inulin inclusion on the lipid oxidation of sausages, TBARS values of the sausages were measured. Regardless of the level and source of inulin utilized the TBARS values of sausage samples increased significantly with increasing frozen storage (Table 7). Domínguez et al. (2019) stated that lipid oxidation in meat and meat products are influenced by storage time; with increasing time the possibility of radicals to cause damage to lipids increases. In addition, the release of iron from heme-proteins gets accelerated with long storage periods and it catalyzes multiple reactions in the initiation and propagation phases of lipid oxidation.

Table 7. Effect of different inulin sources on TBARS values (MDA mg/kg) of low-fat chicken sausages during storage
Period Formulations SEM1)
Control Garlic inulin Commercial inulin
Day 1 0.056Cx 0.039Ey 0.036Cy 0.003
Day 7 0.071Cx 0.053Dy 0.046Cz 0.004
Day 14 0.136Bx 0.088Cy 0.089By 0.009
Day 21 0.171Bx 0.130Bxy 0.099By 0.012
Day 28 0.226Ax 0.154Ay 0.145Ay 0.014
SEM2) 0.017 0.012 0.011

1) Pooled SEM (n=9).

2) Pooled SEM (n=15).

A–E Values in the same column with different superscripts differ significantly (p<0.05).

x–z Values in the same row with different superscripts differ significantly (p<0.05).

Download Excel Table

In addition, both garlic inulin and chicory inulin-added sausages showed lower TBARS values than control sausages throughout the storage period. This could be due to the differences in fat content of the experimental sausages, as shown in the Table 3. It has been previously reported that TBARS values near 0.5 mg/kg sample are indicative of the beginning of oxidation, whereas those above 1.0 mg/kg sample suggest advanced oxidative status (Reitznerová et al., 2017). In the present study, all experimental sausages had TBARS values lower than sensory rancidity detection (<1.0).

Conclusion

This study was conducted with the aim of evaluating the sensory and physicochemical properties of inulin incorporated low-fat chicken sausages. Overall, the findings showed that fat content of the sausages can be reduced from 13.67% (Control sample) to 4.47%–4.85% by incorporating water-based inulin gel to the product formula at the level of 3% inulin. However, 2% garlic inulin contributed to better sensory qualities such as flavour and overall acceptability. In addition, protein, ash and moisture contents of the sausage samples were increased when inulin percentage was increased in the formulation. Hence, garlic inulin can be used to successfully substitute fat in sausage production without altering the meat quality parameters.

Conflicts of Interest

The authors declare no potential conflicts of interest.

Acknowledgements

This study was financially supported by Uva Wellassa University of Sri Lanka (University Capital Grant No: UWU/RG/2018/038). In addition, the authors would like to acknowledge, Ms. Sarangi Karunarathna and Mr. Thilina Jayasinghe from the Meat Science and Research Laboratory, Uva Wellassa University, Sri Lanka for their support.

Author Contributions

Conceptualization: Jayasena DD, Mudannayake DC. Data curation: Jayarathna GN, Jayasena DD, Mudannayake DC. Formal analysis: Jayarathna GN, Jayasena DD, Mudannayake DC. Methodology: Jayarathna GN, Jayasena DD, Mudannayake DC. Validation: Jayasena DD, Mudannayake DC. Investigation: Jayarathna GN. Writing - original draft: Jayarathna GN. Writing - review & editing: Jayarathna GN, Jayasena DD, Mudannayake DC.

Ethics Approval

The design of the sensory evaluation conducted in this study was reviewed and approved by the Research Ethics Review Committee of Uva Wellassa University (No. UWU/REC/2021/003).

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