Oxya chinensis sinuosa extracts protects ARPE-19 cells against oxidative stress via activation of the MAPKs/NF-κB pathway

Bong Sun Kim1,2, Ra-Yeong Choi1, Haeyong Kweon1, Joon Ha Lee1, In-Woo Kim1, Minchul Seo1,*
Author Information & Copyright
1Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.
2Division of Applied Food System, Major in Food Science & Technology, Seoul Women’s University, Seoul 01797, Korea.
*Corresponding Author: Minchul Seo. E-mail:

© Copyright 2024 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Dec 06, 2023 ; Revised: Feb 06, 2024 ; Accepted: Feb 08, 2024

Published Online: Feb 08, 2024


Oxya chinensis sinuosa (OC) is a well-known edible insect. Several researches on the health benefits of OC consumption have been performed to date; however, their effect on eye health remains largely unknown. This study aimed to assess the protective effects of OC extracts on the oxidative stress on the RPE (retinal pigment epithelium) cells. Oxidative damage has been identified as a one of the key regulatory factors in age-related macular degeneration. H2O2-induced ROS production, a well-known oxidative stress factor, can cause cell death in retinal pigment epithelia cells. In this study, we found that three OC extracts effectively prevented H2O2-induced ROS production and subsequent death of ARPE-19 cells in a dose-dependent manner. In addition, the OC extracts inhibited the phosphorylation of MAPKs (mitogen-activated protein kinases) including p38, JNK, and ERK. The OC extracts restored IκBα degradation induced by H2O2, indicating that OC extracts suppressed the activation of nuclear factor-κB. Here we demonstrated the antioxidant and anti-apoptotic effects of the OC extracts on ARPE-19, indicating their potential role in improving eye health. These results suggest that three OC extracts plays a critical role in oxidative stress-induced cell death protects in ARPE-19 cells.

Keywords: Oxya chinensis sinuosa; edible insect; age-related macular degeneration; antioxidant; eye health functional food