Blending three probiotics alleviates loperamide-induced constipation in SD-rats

Ye-JI Jang1, Jin Seok Moon1, Ji Eun Kim1, Dayoung Kim1, Han Sol Choi1, Ikhoon Oh1,*
Author Information & Copyright
1Research Laboratories at ILDONG Pharmaceutical Co., Ltd., Hwaseoung-si 18449, Korea.
*Corresponding Author: Ikhoon Oh. E-mail:

© Copyright 2023 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jul 27, 2023 ; Revised: Sep 04, 2023 ; Accepted: Sep 21, 2023

Published Online: Oct 05, 2023


BIOVITA 3 bacterial species (BIOVITA 3), a probiotic blend powder containing Clostridium butyricum IDCC 1301, Weizmanniacoagulans IDCC 1201, and Bacillus subtilis IDCC 1101, has been used as a food ingredient for gut health. However, its efficacy in improving constipation has not been reported. Therefore, we aimed to investigate the functional effects of oral administration of BIOVITA 3 as well as its component strains alone (at 1.0 x 109 CFU/day) in Sprague-Dawley (SD) rats with loperamide-induced constipation. The study included fecal analysis, gastrointestinal transit ratio, histopathological analysis, short cahin fatty acids (SCFAs), and metagenome analysis. As results, the BIOVITA 3 group showed significant improvements in fecal number, water content, gastrointestinal transit ratio, and thickening of the mucosal layer. In the SCFAs analysis, all probiotic-treated groups showed an increase in total SCFAs compared to the loperamide-constipated group. Changes in microbial abundance and the diversity index of three groups (normal, constipated, and BIOVITA 3) were also defined. Of these, the BIOVITA 3 showed a significant improvement in loperamide-constipated SD-rats. This study suggests the possibility that BIOVITA 3 can be applied as an ingredient in functional foods to relieve constipation.

Keywords: constipation; probiotics; BIOVITA 3 bacterial species; gut microbiota