Fermented Colostrum Whey Upregulates Aquaporin-3 Expression in, and Proliferation of, Keratinocytes via p38/c-Jun N-terminal Kinase Activation

Sang-Ah Seo1,, Hyun-Jung Park1,3,, Min-Gi Han1, Ran Lee1, Ji-Soo Kim1, Ji-Hoo Park1, Won-Young Lee2, Hyuk Song1,*
Author Information & Copyright
1Department of Stem Cell and Regenerative Technology, KIT, Konkuk University, Seoul 05029, Korea.
2Department of Beef & Dairy Science, Korea National College of Agricultures and Fisheries, Jeonbuk 54874, Korea.
3Department of Animal biotechnology, College of Life Science and Natural Resources, Sangji University, Wonju-si 26339, Korea.

† These authors contributed equally to this work.

*Corresponding Author: Hyuk Song, Department of Stem Cell and Regenerative Technology, KIT, Konkuk University, Seoul 05029, Korea. E-mail:

© Copyright 2021 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 02, 2021 ; Revised: Jun 07, 2021 ; Accepted: Jun 12, 2021

Published Online: Jun 28, 2021


Colostrum, which contains various immune and growth factors, aids wound healing by promoting keratinocyte proliferation. Aquaporins (AQPs) are small, hydrophobic membrane proteins that regulate cellular water retention. However, few studies have examined the effect of processed colostrum whey on AQP-3 expression in human skin cells. Here, we investigated the effect of milk, colostrum, fermented milk, and fermented colostrum whey on AQP3 expression in keratinocyte HaCaT cells. Concentrations of 100-400 µg/mL of fermented colostrum whey were found to induce HaCaT cell proliferation. AQP3 was found to be expressed exclusively in HaCaT cells. AQP3 expression was significantly increased in 100 µg/mL fermented colostrum whey-treated cells compared with that in controls. Moreover, fermented colostrum increased p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) phosphorylation, but not ERK1/2 phosphorylation. Thus, our results suggest that fermented colostrum whey increased AQP-3 expression in, and the proliferation of, keratinocytes via JNK and p38 MAPK activation.

Keywords: Colostrum; fermented colostrum; aquaporin-3; keratinocyte; skin disorder

Change of publication charge

As day of June 1, 2021 (based on date of article submission), article processing charges (APC) will be applied to papers accepted after peer review as follows:

Author APC Remark
Member 1,000,000 KR won First and corresponding authors should pay membership fee to Korean Society for Food Science of Animal Resources.
Non-member in Korea 1,200,000 KR won
Other countries except Korea 500 US $ Affiliation of corresponding author

I don't want to open this window for a day.

Special Issue: 67th ICoMST 2021

The 67th International Congress of Meat Science and Technology (ICoMST) is currently accepting abstracts.

The deadline for submitting abstracts has been extended to April 15th, 2021, and FSAR will apply a 50% publishing fee discount to this special issue.

For more information, please check the website below.


I don't want to open this window for a day.