Short Communication

Purification of pig muscle stem cells using magnetic-activated cell sorting (MACS) based on the expression of CD29

Kwang-Hwan Choi1,, Minsu Kim1,, Ji Won Yoon1, Jinsol Jeong1, Minkyung Ryu1, Cheorun Jo1,2,*, Chang-Kyu Lee1,2,*
Author Information & Copyright
1Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
2Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea.

† These authors contributed equally to this work.

*Corresponding Author: Cheorun Jo, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea. E-mail: cheorun@snu.ac.kr.
*Corresponding Author: Chang-Kyu Lee, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea. E-mail: leeck@snu.ac.kr.

© Copyright 2020 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jun 03, 2020 ; Revised: Jul 07, 2020 ; Accepted: Jul 09, 2020

Published Online: Jul 15, 2020

Abstract

The muscle stem cells of domestic animals are of interest to researchers in the food and biotechnology industries for the production of cultured meat. For producing cultured meat, it is crucial for muscle stem cells to be efficiently isolated and stably maintained in vitro on a large scale. In the present study, we aimed to optimize the method for the enrichment of pig muscle stem cells using a magnetic-activated cell sorting (MACS) system. Pig muscle stem cells were collected from the biceps femoris muscles of 14-d-old pigs of three breeds (Landrace × Yorkshire × Duroc (LYD), Berkshire, and Korean native pigs) and cultured in skeletal muscle growth medium-2 (SkGM-2) supplemented with epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580). Approximately 60% of total cultured cells were nonmyogenic cells in the absence of purification in our system, as determined by immunostaining for CD56 and CD29, which are known markers of muscle stem cells. Interestingly, following MACS isolation using the CD29 antibody, the proportion of CD56+/CD29+ muscle stem cells was significantly increased (91.5 ± 2.40%), and the proportion of CD56 single-positive nonmyogenic cells was dramatically decreased. Furthermore, we verified that this method worked well for purifying muscle stem cells in the three pig breeds. Accordingly, we found that CD29 is a valuable candidate among the various marker genes for the isolation of pig muscle stem cells and developed a simple sorting method based on a single antibody to this protein.

Keywords: pig; muscle stem cells; purification; magnetic-activated cell sorting (MACS); CD29


Journal Title Change

We announce that the title of our journal and related information were changed as below from January, 2019.

 

Before (~2018.12)

After (2019.01~)

Journal Title

Korean Journal for Food Science of Animal Resources

Food Science of Animal Resources

Journal Abbreviation

Korean J. Food Sci. An.

Food Sci. Anim. Resour.

eISSN

2234-246X

2636-0780

pISSN

1225-8563

2636-0772

Journal Homepage

http://www.kosfaj.org

Same

JCR Citation Indexing

SCIE

SCIE


I don't want to open this window for a day.