Oral Administration of Bifidobacterium lactis Ameliorates Cognitive Deficits in Mice Intracerebroventricularly Administered Amyloid Beta via Regulation the Activation of Mitogen-activated Protein Kinases

Jong Kyu Choi1, Oh Yun Kwon1,2, Seung Ho Lee1,2,*
Author Information & Copyright
1Department of Nano-Bioengineering, Incheon National University, Incheon 22012, Korea.
2Institute of New Drug Development, Incheon National University, Incheon 22012, Korea.
*Corresponding Author: Seung Ho Lee. E-mail:

© Copyright 2024 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Dec 28, 2023 ; Revised: Jan 04, 2024 ; Accepted: Jan 05, 2024

Published Online: Jan 09, 2024


Probiotics are functional microorganisms that exhibit various biological activities, such as allergic reactions, inflammation, and aging. The aim of this study is to evaluate the effects of Bifidobacterium lactis CBT BL3 (BL) on the amyloid beta (Ab)-mediated cognitive impairments. Oral administration of live BL to intracerebroventricularly Ab-injected mice significantly attenuated short- and long-term memory loss estimated using the Y-maze and Morris water maze tests. We found that expression of apoptosis-related proteins such as caspase-9, caspase-3, and cleaved poly (ADP-ribose) polymerase was significantly elevated in the brain tissues of Ab-injected mouse brains when compared to that of the control mouse group. Interestingly, these expression levels were significantly decreased in the brain tissue of mice fed BL for 6 weeks. In addition, the abnormal over-phosphorylation of mitogen-activated protein kinases (MAPKs) such as ERK1/2, p38 MAPK, and JNK in the brain tissue of intracerebroventricularly Ab-injected mice was significantly attenuated by oral administration of BL. Taken together, the results indicate that Ab-induced cognitive impairment may be ameliorated by the oral administration of BL by controlling the activation of MAPKs/apoptosis in the brain. This study strongly suggests that BL can be developed as a functional probiotic to attenuate Ab-mediated cognitive deficits.

Keywords: Bifidobacterium lactis; cognitive deficits; Alzheimer’s disease; probiotics