Lactiplantibacillus plantarum LM1001 improves digestibility of branched-chain amino acids in whey proteins and promotes myogenesis in C2C12 myotubes

Youngjin Lee1, Yoon Ju So1, Woo-Hyun Jung1, Tae-Rahk Kim1, Minn Sohn1, Yu-Jin Jeong2, Jee-Young Imm2,*
Author Information & Copyright
1Microbiome R&D Center,Lactomason Co. Ltd., Jinju 52840, Korea
2Department of Foods and Nutrition, Kookmin University, Seoul 02707, Korea
*Corresponding Author: Jee-Young Imm. E-mail:

© Copyright 2024 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 11, 2024 ; Revised: May 02, 2024 ; Accepted: May 03, 2024

Published Online: May 22, 2024


Lactiplantibacillus plantarum is a valuable potential probiotic species with various proven health-beneficial effects. L. plantarum LM1001 strain was selected among ten strains of L. plantarum based on proteolytic activity on whey proteins. L. plantarum LM1001 produced higher concentrations of total free amino acids and branched-chain amino acids (BCAA: Ile, Leu, and Val) than other L. plantarum strains. Treatment of C2C12 myotubes with whey protein culture supernatant (1%, 2% and 3%, v/v) using L. plantarum LM1001 significantly increased the expression of myogenic regulatory factors, such as Myf-5, MyoD, and myogenin, reflecting the promotion of myotubes formation (p<0.05). L. plantarum LM1001 displayed β-galactosidase activity but did not produce harmful b-glucuronidase. Thus, the intake of whey protein together with L. plantarum LM1001 has the potential to aid protein digestion and utilization.

Keywords: Lactiplantibacillus plantarum LM1001; digestibility; branched-chain amino acids; myogenesis; C2C12 myotubes