Effects of Lactobacillus reuteri MG5346 on RANKL-induced osteoclastogenesis and ligature-induced experimental periodontitis rats

Yu-Jin Jeong1, Jae-In Jung1, YongGyeong Kim2, Chang-Ho Kang2, Jee-Young Imm1,*
Author Information & Copyright
1Department of Foods and Nutrition, Kookmin University, Seoul 02707, Korea
2Mediogen, Co., Ltd., Jecheon 27159, Korea
*Corresponding Author: Jee-Young Imm, Department of Foods and Nutrition, Kookmin University, Seoul 02707, Korea. E-mail:

© Copyright 2022 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 06, 2022 ; Revised: Nov 13, 2022 ; Accepted: Nov 18, 2022

Published Online: Nov 18, 2022


Effects of culture supernatants of Lactobacillus reuteri MG5346 (CS-5346) on receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis were examined. CS-MG5346 treatment up to 400 μg/mL significantly reduced tartrate-resistant acid-phosphatase (TRAP) activity, the phenotype biomarker of osteoclast, without affecting cell viability. CS-MG5346 inhibited the expression of osteoclast specific transcriptional factors (c-fos and nuclear factor-activated T cells c1) and their target genes (TRAP, cathepsin, and matrix metallo-proteinase-9) in a dose-dependent manner (p < 0.05). The administration of L. reuteri MG5346 (2 × 108 CFU/day) for 8 wks significantly improved furcation involvement, but no difference was observed in alveolar bone loss in ligature-induced experimental periodontitis rats. The elevated RANKL/osteoprotegerin ratio, the biomarker of periodontitis, was significantly lowered in the gingival tissue by administration of L. reuteri MG5346 (p < 0.05). L. reuteri MG5346 showed excellent stability in simulated stomach and intestinal fluids and did not have antibiotic resistance. Based on the results, L. reuteri MG5346 has the potential to be a promising probiotic strain for oral health.

Keywords: Lactobacillus reuteri MG5346; culture supernatant; osteoclastogenesis; osteoclast specific gene expression; ligature-induced experimental periodontitis