Comparison of functional properties of blood plasma collected from black goat and Hanwoo cattle

Shine Htet Aung1,2, Edirisinghe Dewage Nalaka Sandun Abeyrathne1,3, Mahabbat Ali1,4, Dong Uk Ahn5, Young-Sun Choi6, Ki-Chang Nam1,*
Author Information & Copyright
1Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea.
2Department of Zoology, Kyaukse University, Kyaukse 05151, Myanmar.
3Department of Animal Science, Uva Wellassa University, Badulla 90000, Sri Lanka.
4Department of Animal Production and Management, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh.
5Department of Animal Science, Iowa State University, Ames 50011, United States.
6Jeollanamdo Agricultural Research and Extension Services, Gangin 59213, Korea.
*Corresponding Author: Ki-Chang Nam, Department of Animal Science & Technology, Sunchon National University, Suncheon 57922, Korea. Phone: +82-61-750-3231. E-mail:

© Copyright 2022 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 19, 2022 ; Revised: Sep 19, 2022 ; Accepted: Sep 21, 2022

Published Online: Oct 04, 2022


Slaughterhouse blood is a by-product of animal slaughter that can be a good source of animal protein. This research purposed to examine the functional qualities of the blood plasma from Hanwoo cattle, black goat, and their hydrolysates. Part of the plasma was hydrolyzed with proteolytic enzymes (Bacillus protease, papain, thermolysin, elastase, and α-chymotrypsin) to yield bioactive peptides under optimum conditions. The levels of hydrolysates were evaluated by 15% SDS-PAGE gel electrophoresis. The antioxidant, metal-chelating, and angiotensin I-converting enzyme (ACE) inhibitory properties of intact blood plasma and selected hydrolysates were investigated. Accordingly, two plasma hydrolysates by protease (pH 6.5/ 55oC/ 3 h) and thermolysin (pH 7.5/ 37oC/ 3-6 h) were selected for analysis of their functional properties. In the oil model system, only goat blood plasma had lower levels of thiobarbituric acid reactive substances (TBARS) than the control. The diphenyl picrylhydrazyl (DPPH) radical scavenging activity was higher in cattle and goat plasma than in proteolytic hydrolysates. Iron-chelating activities increased after proteolytic degradation except for protease-treated cattle blood. Copper-chelating activity was excellent in all test samples except for the original bovine plasma. As for ACE inhibition, only non-hydrolyzed goat plasma and its hydrolysates by thermolysin showed ACE inhibitory activity (9.865.03% and 21.773.74%). In conclusion, goat plasma without hydrolyzation and its hydrolysates can be a good source of bioactive compounds with functional characteristics, whereas cattle plasma has a relatively low value. Further studies on the molecular structure of these compounds are needed with more suitable enzyme combinations.

Keywords: Blood by-product; Enzyme hydrolysis; Antioxidant; Metal chelating; ACE inhibitory