Table 2. IPTG induction conditions for each Escherichia coli BL21 (pET22b-ldhLs)

Strain	Condition
E. coli BL21 (pET22b-ldhL1)	16°C, 170 rpm, 10 h
E. coli BL21 (pET22b-ldhL2)	37°C, 150 rpm, 4 h
E. coli BL21 (pET22b-ldhL3)	37°C, 150 rpm, 4 h
E. coli BL21 (pET22b-ldhL4)	37°C, 225 rpm, 4 h