Table 1. Bacteria strains and plasmids used in this study

Strains or plasmid Characteristics Source or reference
Strain
Lacticaseibacillus casei HY2782 Wild-type strain hy Co., Ltd.
Escherichia coli DH5α φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44λ-thi-1 BioFACT
E. coli BL21 (DE3) F- ompT hsdSB(rB-mB-) gal dcm (DE3) Invitrogen
Plasmid
 pGEM-T Easy Cloning vector, Ampr Promega
 pET22b (+) Expression vector (His-Tag), Ampr Merch KGaA
 pJE1 pGEM-T Easy containing 951 bp PCR fragment corresponding to the ldhL1 gene This study
 pJE2 pGEM-T Easy containing 903 bp PCR fragment corresponding to the ldhL2 gene This study
 pJE3 pGEM-T Easy containing 993 bp PCR fragment corresponding to the ldhL3 gene This study
 pJE4 pGEM-T Easy containing 918 bp PCR fragment corresponding to the ldhL4 gene This study
 pET22b-ldhL1 pET22b (+) containing 939 bp NdeI-XhoI fragment derived from pJE1 This study
 pET22b-ldhL2 pET22b (+) containing 891 bp NdeI-XhoI fragment derived from pJE2 This study
 pET22b-ldhL3 pET22b (+) containing 981 bp NdeI-XhoI fragment derived from pJE3 This study
 pET22b-ldhL4 pET22b (+) containing 906 bp NdeI-XhoI fragment derived from pJE4 This study