Table 2. Primers used for qRT-PCR

Gene	Forward primer (5’→3’)	Reverse primer (5’→3’)	Product size (bp)
Nf-κb	ATGTGCATCGGCAAGTGG	CAGAAGTTGAGTTTCGGGTAG	294
ZO-1	GATGTTTATGCGGACGGTGG	CATTGCTGTGCTCTTAGCGG	132
Occludin	AAGTCAACACCTCTGGTGC	CCTTCGTGGGAGCCCTTTT	130
β-actin	GGCTGTATTCCCCTCCATCG	CCAGTTGGTAACAATGCCATGT	154

qRT-PCR, quantitative real-time reverse transcription polymerase chain reaction; Nf-κb, NF-kappaB; ZO-1, zonula occludens-1.