Table 2. The primer sequences used for quantitative real-time PCR

Gene	Primer sequence (5’–3’)	Reference
GAPDH	F: ACTCTGGCAAAGTGGATGTTGTC	Li et al. (2019)
GAPDH	R: GCATCACCCCACTTGATGTTG	Li et al. (2019)
PAX7	F: TGCCCTCAGTGAGTTCGATT	Li et al. (2019)
PAX7	R: CGGGTTCTGACTCCACATCT	Li et al. (2019)
MyoG	F: AGAAGGTGAATGAAGCCTTCGA	de Las Heras-Saldana et al. (2019)
MyoG	R: GCAGGCGCTCTATGTACTGGAT	de Las Heras-Saldana et al. (2019)
MRF4	F: GGTGGACCCCTTCAGCTACAG	Shibata et al. (2006)
MRF4	R: TGCTTGTCCCTCCTTCCTTGG	Shibata et al. (2006)
MHC1	F: CCCACTTCTCCCTGATCCACTAC	Ahn et al. (2020)
MHC1	R: TTGAGCGGGTCTTTGTTTTTCT	Ahn et al. (2020)

PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PAX7, paired box 7; MyoG, myogenin; MRF4, muscle regulatory factor 4; MHC1, myosin heavy chain 1.