Table 1. qPCR reaction condition

Stage	Step		Temp (°C)	Time
Holding	1. Initial denaturation		95	5 min
	2. Denaturation		95	30 s
Cycling (repeat 40 cycles)	3. Annealing	GAPDH	55	30 s
		PAX7	53	30 s
		MRF4	56	30 s
		MHC1	54	30 s
	4. Extension		72	90 s
Melt curve	5. Final extension		95	15 s
			60	1 min
			95	15 s
	6. Hold		4	∞

qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PAX7, paired box 7; MRF4, muscle regulatory factor 4; MHC1, myosin heavy chain 1.