Table 1. The percentage composition of fatty acids in mealworms extracted using n-hexane, ethyl acetate (EtOAc), ethanol (EtOH), and isopropanol (IPA)

Peak	Formula	Name of compounds	EtOAc	EtOH	IPA	n-Hexane
1	C10:1	Decanoic acid, methyl ester	0.02±0.003^a	0.02±0.003^a	0.02±0.00^a	0.03±0.006^a
2	C12:0	Dodecanoic acid, methyl ester	0.51±0.1^ab	0.20±0.008^c	0.32±0.14^b	0.55±0.023^a
3	C13:0	Tridecanoic acid, methyl ester	0.11±0.00^a	0.26±0.13^a	0.10±0.00^a	0.13±0.003^a
4	C14:1	Methyl myristoleate	0.43±0.003^a	0.16±0.00^b	0.36±0.03^a	0.43±0.067^a
5	C16:1	Methyl E-11-tetradecenoate	0.22±0.007^b	0.10±0.006^c	0.21±0.00^b	0.26±0.007^a
6	C16:1	Methyl Z-11-tetradecenoate	0.01±0.007^b	0.02±0.003^ab	0.02±0.00^ab	0.04±0.003^a
7	C14:0	Methyl tetradecanoate	7.13±0.053^b	3.37±0.046^d	6.42±0.003^c	7.33±0.037^a
8	C14:1	Tetradecanoic acid, 12-methyl-, methyl ester, (S)-	0.03±0.006^a	0.02±0.009^a	0.05±0.012^a	0.04±0.013^a
9	C16:1	7-Hexadecenoic acid, methyl ester, (E)-	0.05±0.003^b	0.03±0.00^c	0.05±0.00^b	0.06±0.003^a
10	C16:1	7-Hexadecenoic acid, methyl ester, (Z)-	0.02±0.007^ab	0.01±0.003^b	0.03±0.00^ab	0.04±0.007^a
11	C16:0	Pentadecanoic acid, methyl ester	0.13±0.017^b	0.07±0.003^c	0.15±0.00^ab	0.18±0.007^a
12	C16:2	7,10-Hexadecadienoic acid, methyl ester	0.37±0.003^b	0.14±0.003^c	0.34±0.00^b	0.45±0.013^a
13	C18:1	9-Hexadecenoic acid, methyl ester, (Z)-	1.77±0.03^b	0.83±0.00^d	1.67±0.00^c	2.09±0.003^a
14	C16:1	Methyl hexadec-9-enoate	3.01±0.007^b	1.27±0.009^d	2.78±0.00^c	3.41±0.01^a
15	C16:0	Hexadecanoic acid, methyl ester	22.66±0.14^b	16.35±0.12^d	21.13±0.00^c	24.62±0.14^a
16	C17:1	cis-10-Heptadecenoic acid, methyl ester	0.23±0.013^b	0.07±0.012^c	0.26±0.00^ab	0.29±0.013^a
17	C17:0	Heptadecanoic acid, methyl ester	0.13±0.013^c	0.34±0.003^a	0.19±0.003^b	0.21±0.003^b
18	C18:2	9,12-Octadecadienoic acid (Z,Z)-, methyl ester	24.70±0.25^d	36.86±0.31^a	27.93±0.08^c	29.78±0.09^b
19	C18:1	9-Octadecenoic acid (Z)-, methyl ester	35.10±0.09^a	26.69±0.37^c	32.22±0.00^b	25.23±0.033^d
20	C20:0	Methyl stearate	2.85±0.091^c	9.06±0.084^a	3.37±0.00^b	3.27±0.07^b
21	C18:1	6-Octadecenoic acid, methyl ester, (Z)-	0.01±0.00^a	0.11±0.038^a	0.09±0.077^a	0.07±0.063^a
22	C18:1	11-Octadecenoic acid, methyl ester	0.02±0.00^b	0.16±0.052^a	0.03±0.00^b	0.04±0.00^b
23	C19:1	cis-10-Nonadecenoic acid, methyl ester	0.06±0.003^c	0.14±0.003^b	0.23±0.00^a	0.13±0.003^b
24	C19:0	Nonadecanoic acid, methyl ester	0.02±0.003^c	0.19±0.006^a	0.06±0.00^b	0.06±0.003^b
25	C20:3	Methyl 5,11,14-eicosatrienoate	0.02±0.003^b	0.11±0.009^b	0.45±0.00^a	0.43±0.63^a
26	C20:1	cis-11-Eicosenoic acid, methyl ester	0.28±0.033^b	3.41±0.68^a	0.15±0.00^b	0.11±0.05^b
27	C20:1	Eicosanoic acid, methyl ester	0.11±0.00^c	0.00±0.00^d	1.38±0.00^a	0.72±0.003^b
	SFA (%)		33.9	29.98	32.08	36.8
	MUFA (%)		41.37	33.04	39.55	32.99
	PUFA (%)		25.07	37.0	28.27	30.23
	PUFA/SFA		0.73	1.23	0.88	0.82

The data is presented as means±SD, with a sample size of n=3.
Letters accompanying the means signify statistically significant differences (p<0.05) among the extraction solvents.
SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids.