Table 2. Studies on non-destructive methods for inspecting frozen meat properties

References Sample Techniques Determination Measurements Calibration set (training set) Prediction set (validation or test set)
Imaging-based techniques
Pu et al. (2015) Pork Hyperspectral imaging (HSI) (400–1,000 nm) Classification of fresh and repeated frozen-thawed meat Classification CC%=93.14% CC%=90.91%
Xie et al. (2015) Pork HSI (400–1,000 nm) Prediction of color and water-holding capacity CIE L* - r2=0.907
Cooking loss - r2=0.845
CIE b* - r2=0.814
Drip loss - r2=0.762
CIE a* - r2=0.716
Cheng et al. (2018) Pork HSI (1,000–2,200 nm) Prediction of tertiary protein structure and enzyme activity Surface hydrophobicity r2C=0.893 RMSEC=1.576 r2P=0.896 RMSEP=1.549
Ca2+-ATPase activity r2C=0.896 RMSEC=0.014 r2P=0.879 RMSEP=0.015
Cheng et al. (2019) Pork HSI (1,000–2,200 nm) Prediction of secondary protein structure α-Helix fraction in actomyosin r2C=0.789 RMSEC=2.170% r2P=0.836 RMSEP=1.737%
Cheng et al. (2022b) Pork Fluorescence-HSI Prediction of protein oxidation Carbonyl content r2C=0.9305 RMSEC=0.1011 r2P=0.9275 RMSEP=0.0812
Total sulfhydryl content r2C=0.9550 RMSEC=1.6096 r2P=0.9512 RMSEP=1.2979
Cheng et al. (2023b) Pork HSI (400–1,002 nm) Prediction of lipid and protein oxidation TBARS r2C=0.9889 RMSEC=0.0182 r2P=0.9724 RMSEP=0.0227
Carbonyl content r2C=0.9824 RMSEC=0.0530 r2P=0.9602 RMSEP=0.0702
Cheng et al. (2023a) Pork HSI (400–1,002 nm) Prediction of lipid oxidation TBARS r2C =0.9830 RMSEC=0.0153 r2P =0.9697 RMSEP=0.0184
Fluorescence-HSI r2C=0.9833 RMSEC=0.0140 r2P =0.9726 RMSEP=0.0182
Wei et al. (2024) Beef HSI (328–1,115 nm) Prediction of freezing point and water mobility Freezing point r2C=0.82 RMSEC=0.12 r2P=0.76 RMSEP=0.11
P21 r2C=0.95 RMSEC=0.38 r2P=0.80 RMSEP=0.67
P22 r2C=0.96 RMSEC=0.39 r2P=0.84 RMSEP=0.71
Jeong et al. (2025) Pork HSI (402–1,002 nm) Classification of frozen storage conditions and thawing loss Frozen storage conditions CC%=83.20% CC%=81.82%
Thawing loss CC%=93.36% CC%=91.92%
Spectroscopy-based techniques
Gudjónsdóttir et al. (2019) Atlantic mackerel Low-field nuclear magnetic resonance (LF-NMR) Prediction of water content, total lipids, water-holding capacity Water content - r2=0.799
Total lipids - r2=0.760
Water-holding capacity - r2=0.691
Chen et al. (2020) Beef Raman spectroscopy Prediction of texture properties Hardness (g) r2C=0.82 RMSEC=11.9 r2P=0.82 RMSEP=12.8
Tenderness (N) r2C=0.83 RMSEC=2.78 r2P=0.81 RMSEP=2.57
Chewiness (g.s) r2C=0.91 RMSEC=625 r2P=0.80 RMSEP=942
Firmness (g) r2C=0.91 RMSEC=8.70 r2P=0.81 RMSEP=11.5
Springiness (%) r2C=0.71 RMSEC=2.75 r2P=0.53 RMSEP=2.26
Chen et al. (2023) Beef Raman spectroscopy Prediction of water content and water-holding capacity Thawing loss r2C=0.994 RMSEC=0.640 r2P=0.971 RMSEP=1.436
Water content r2C=0.966 RMSEC=0.450 r2P=0.928 RMSEP=0.582
Ropodi et al. (2018) Beef Fourier-transform infrared (FTIR) Classification of fresh and frozen beef at –20°C (7 and 32 d) Classification CC%=100% CC%=93.33%
Cáceres-Nevado et al. (2021) Pork Near-infrared (NIR) Classification of fresh and frozen pork at –20°C Classification CC%=99.35% CC%=100%
CC, classification rates; RMSEC, root mean standard error for calibration; RMSEP, root mean square error of prediction.