Table 1. Previous studies on the modification of physicochemical properties in meat by frozen storage conditions

References Materials Freezing conditions Analysis content Measurements Results
Zhu et al. (2022) Pork –18°C, –40°C, –80°C, and liquid nitrogen cryogen freezing Protein oxidation Carbonyl content The highest carbonyl content was observed under freezing at –18°C during the entire storage period
Lipid oxidation Thiobarbituric acid reactive substance (TBARS) The highest TBARS was observed under freezing at –18°C during the entire storage period
Zhang et al. (2023) Beef –20°C (30, 60, 120, or 180 d) Tertiary structure Surface hydrophobicity The surface hydrophobicity decreased after 120 d
Qian et al. (2022) Beef –20°C, –40°C, and –80°C (12, 24, and 48 h) Protein functionality Protein solubility The protein solubility during frozen storage at –20°C was the lowest among all storage durations
Tertiary structure Myofibrillar proteins (MPs) surface hydrophobicity Frozen storage at –20°C increases the surface hydrophobicity at freezing temperatures
Secondary structure Fourier trans-formation infrared spectrum (FTIR) Higher freezing rates and shorter freezing times decrease α-helix and increase β-turn and random coil of MP samples.
Microstructure Light microscope The highest destruction of muscle cells was observed under frozen storage at –20°C.
Soyer et al. (2010) Chicken –7°C, –12°C, and –18°C (1, 2, 3, 4, 5, and 6 mon) Lipid oxidation TBARS The lowest TBARS level was observed under frozen storage at –18°C
Protein oxidation Carbonyl contents The lowest carbonyl content was observed under frozen storage at –18°C
Medić et al. (2018) Pork –18°C (fresh, 3, 6, 12, 15, and 18 mon) Lipid oxidation TBARS The highest TBARS value of ham and loins was observed after 12 mon, whereas the belly rib showed the highest TBARS value after 15 mon.
Li et al. (2021) Pork –18°C (fresh, 30, 60, 90, and 180 d) Protein oxidation Carbonyl contents Freezing at –18°C causes increased carbonyl contents during extended storage periods.
Tertiary structure Tryptophan fluorescence intensity Freezing at –18°C causes increased exposure of the buried tryptophan residues during extended storage periods
Zequan et al. (2019) Pork –18°C (fresh, 3, 6, 9, 12, 15, and 18 weeks) Muscle structure Light microscope The muscle tissues shrink after 9 weeks
Muela et al. (2015) Lamb –18°C (fresh, 1, 9, 15, and 21 mon) Water-holding capacity Thawing loss The thawing loss is gradually increased
pH pH The pH is gradually decreased