Table 1. Microbial relative abundance comparison in MPC2 and MPC20 adjusted for nNOS and corticosterone

	Effect size (Log2 FC)1)	SE	p-value	FDR2)
Ileum
Bifidobacterium	–1,230	250	0.0001	0.0127
Cecum
Prevotellaceae_NK3B31_group	–680	75.9	<0.0001	<0.0001
Odoribacter	–148	35.1	0.0004	0.0117
Faecalibaculum	–546	146	0.0013	0.0261
Bifidobacterium	–295	85.2	0.0023	0.0382
Colon
Faecalibaculum	–865	124	<0.0001	0.0001
Prevotellaceae_NK3B31_group	–386	63.7	<0.0001	0.0004
Bifidobacterium	–389	89.7	0.0004	0.0112
Bacteroides	–770	183	0.0003	0.0112

Multiple linear regression analysis was conducted using MaAsLin2 with nNOS and corticosterone as covariates. The effect size indicates the change in microbial relative abundance of MPC20 compared to that of MPC2.
Results with a statistically significant FDR (p<0.05) are shown in the table.
MPC2, two mice per cage; MPC20, twenty mice per cage; nNOS, neuronal nitric oxide synthase; FDR, false discovery rate.