Table 1. Introduction of materials according to scaffold production method

Production method Materials Cells Introduction References
Electrospinning Textured soy protein Bovine satellite cell A step forward for the production of cell-based meat as food Ben-Arye et al., 2020
Electrospinning Polycaprolactone Endothelial cell Developing new scaffolds by investigating the interactions between endothelial cells, starch, and polyfibers Santos et al., 2007
Electrospinning Cellulose fibers - Systematic study of electrospinning conditions and application as reinforcing fiber for biocomposites Han et al., 2008
Molding Soybean powder and gelatin C2C12 and 3T3-L1 cells Manufacture cultured meat with a muscle-like texture by adding pre-fat cells that produce mass-produced cultured meat Li et al., 2022
Molding Polydimethylsiloxane and naringenin Porcine satellite cells Development of an efficient and innovative cultured meat production system through upregulation of signal transduction Yan et al., 2022
Molding Sodium alginate Murine myoblast C2C12 cell line Utility of a dual cross-linked alginate hydrogel system to support in vitromeat growth Tahir and Floreani, 2022
3D printing Salecan and κ-carrageenan Mouse fibroblast cells A new strategy for fabricating and optimizing polysaccharide-based hydrogel scaffolds Qi et al., 2020
3D printing Sol-gel transition and ionic gelation Mouse C2C12 Analyzing the adhesion of gellan gum and developing a new support accordingly Koivisto et al., 2019
3D printing Soy protein isolate Primary bovine satellite cell Great potential for research on cultivated meat through the use of peas, which have low allergenicity Ianovici et al., 2022
Decellularization Spinach Primary bovine satellite cell A cost-effective and environmentally friendly scaffold, potentially accelerating the development of laboratory-grown meat Jones et al., 2021
Decellularization Fresh whole jackfruit Primary bovine satellite cell Proposing a new closed bioreactor system for cellular agriculture products Perreault et al., 2023
Decellularization Grass blade Murine C2C12 myoblasts Presents the need for the development of inexpensive and sustainable support materials and structures Allan et al., 2021
Freeze-drying Gellan gum and guar gum Mouse fibroblast cell (L929) Development of a new scaffold that confirmed the cytotoxicity and cell adhesion of the scaffold Anandan et al., 2019
Freeze-drying Hydrolyzed collagen - Focus on using food-grade materials to ensure commercial availability of developed collagen ink formulations Koranne et al., 2022
Freeze-drying Collagen from turkey tendons Skeletal muscle satellite cells A major challenge for successful cultured meat production is the need for large quantities of skeletal muscle satellite cells Andreassen et al., 2022