Table 3. The optimized formulation based on the constraints applied to the significant variables and the corresponding antibacterial results are actual experimental values

Variable		Formulation 1
A: Ecklonia cava		58.40
B: Psidium guajava		39.68
C: Paeonia japonica (Makino) Miyabe & Takeda		1.92
Total (A+B+C)		100.00
Desirability		0.97
MIC (mg/mL)1)	Escherichia coli	2.50
	Salmonella spp.	1.25
	Listeria monocytogenes	2.50
MBC (mg/mL)1)	E. coli	2.50
	Salmonella spp.	2.50
	L. monocytogenes	5.00

Average value measured through actual three repeated experiments.
MIC, minimum inhibitory concentration; MBC, minimum bactericidal concentration.