Table 1. List of primer sequence used for quantitative real-time polymerase chain reaction (qRT-PCR) analysis in present study

Gene symbol	Primer sequences		Annealing temperature (°C)
IL-6	Forward	5’-GAGGATACCACTCCCAACAGACC-3’	56
	Reverse	5’-AAGTGCATCATCGTTGTTCATACA-3’
TNF-a	Forward	5’-TGCCTATGTCTCAGCCTCTTC-3’	56
	Reverse	5’-GAGGCCATTTGGGAACTTCT-3’
Atrogin-1	Forward	5’-CGACCTGCCTGTGTGCTTA-3’	60
	Reverse	5’-GTCCACGTCAGCAATCATCC-3’
MuRF-1	Forward	5’-TGCCTACTTGCTCCTTGTGC-3’	60
	Reverse	5’-CACCAGCATGGAGATGCAGT-3’
GAPDH	Forward	5’-CACTCACGGCAAATTCAACGGCAC-3’	56, 60
	Reverse	5’-GACTCCACGACATACTCAGCAC-3’

IL, interleukin; TNF, tumor necrosis factor; MuRF-1, muscle RING finger protein-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.