Table 2. Comparison of the iELISA developed in this study with other commercial kits

Variable	Commercial kit No. 11)	Commercial kit No. 2	Commercial kit No. 3	This study
Detection technique	Lateral flow assay	Lateral flow assay	Sandwich ELISA	Indirect ELISA
Target	Raw meat, processed meat, fat, oil, gelatin, prior sending	Porcine serum albumin	Cooked pork meat	TSSP in pork fat
Step required	2	2	4	5
Limit of detection	1%–2%	0.5%	1%	0.015%

Commercial kits No. 1: Porcine Trace Rapid Test Kit Fat (#RHAL01-03-020) by 7FoodPillars, No. 2: XEMATest Pork fat/blood (#X316) by XEMATest, and No. 3: ELISA-TEK™ Cooked Meat Pork Species Kit (#510621) by R-Biopharm AG.
iELISA, indirect enzyme-linked immunosorbent assay; TSSP, thermal stable-soluble protein.