Table 1. The overall effect of coating application and storage period on the microbiological and physico-chemical properties of rainbow trout fillets

Factor	n	TMAB (Log CFU/g)	Psychrotrophic bacteria (Log CFU/g)	pH	TBARS (μmol MDA/kg)	TVB-N (mg/100 g)
Coating application (CA)
Control	28	6.08±1.64^c	5.98±1.73^c	6.56±0.07^c	27.75±25.65^a	21.69±8.25^b
CH	28	4.12±1.04^b	4.02±1.14^a	6.41±0.08^a	26.48±23.22^a	17.49±1.24^a
CHT	28	4.18±1.09^b	4.28±0.86^b	6.45±0.08^b	26.66±23.90^a	17.05±1.25^a
CHTRA	28	3.91±1.11^a	4.14±0.93^ab	6.43±0.09^ab	31.26±29.68^b	17.34±1.30^a
Significance		^**	^**	^**	^*	^**
Storage period (SP)
0 d	16	3.22±0.70^a	3.39±0.58^a	6.50±0.11^b	3.13±2.15^a	15.16±0.76^a
3 d	16	3.44±0.74^a	3.47±0.60^a	6.48±0.10^b	4.77±2.68^a	16.62±0.87^b
6 d	16	3.81±0.72^b	3.96±0.62^b	6.42±0.09^a	9.79±1.93^b	17.12±0.58^b
9 d	16	4.30±1.08^c	4.33±1.08^c	6.47±0.06^b	19.79±4.73^c	17.32±0.53^b
12 d	16	5.12±1.15^d	4.96±1.15^d	6.41±0.09^a	33.06±6.70^d	18.27±1.81^c
15 d	16	5.68±1.32^e	5.68±1.27^e	6.48±0.09^b	53.19±9.51^e	20.45±4.21^d
18 d	16	6.44±1.32^f	6.44±1.33^f	6.48±0.11^b	72.34±12.91^f	23.80±9.07^e
Significance		^**	^**	^**	^**	^**
CA×SP		^**	^**	^**	^**	^**

Mean±SD.
Different letters indicate statistical difference (p<0.05) in each column. ^* p<0.05; ^** p<0.01.
TMAB, total aerobic mesophilic bacteria; TBARS, thiobarbituric acid reactive substances; MDA, malondialdehyde; TVB-N, total volatil basic nitrogen; CH, chitosan; CHT, chitosan+TiO₂ nanoparticles; CHTRA, chitosan+TiO₂ nanoparticles+rosmarinic acid.