Table 2. Effect of enzyme, pH, temperature, and solvent treatment on the activity of the recombinant agilicin C7

Treatment	Residual activity (%)1)
Temperature (°C)
4	100
25	100
37	100
50	100
60	100
80	90
100	80
121	ND
pH
1	100
2	100
3	100
4	100
5	100
6	100
7	100
8	100
9	95
10	95
Enzyme (5 mg/mL)
α-Amylase	ND
α-Chymotrypsin	ND
Proteinase-K	ND
Trypsin	ND
Pepsin	ND
Lipase	90
Solvent
Acetone	90
Acetonitrile	90
Methanol	90
Ethanol	95
Chloroform	100
Isopropanol	100

Residual activity (%) is measured by the diameter of the inhibition zones compared to untreated control.
ND, not detected.