Table 1. Antimicrobial spectrum of recombinant agilicin C7

Indicator organism	Inhibition zone	Media
Gram-negative
Pseudomonas aeruginosa KCCM 11266	−	NB
Salmonella gallinarum KVCC-BA0700722	−	NB
Shigella flexneri KCCM 40414	−	NB
Salmonella typhimurium KCCM 40253	−	NB
Escherichia coli K88	−	LB
Klebsiella pneumoniae KCCM11418	−	NB
Salmonella enteritidis KCCM12021	−	NB
E. coli DH5α (Cloning host)	−	LB
E. coli BL21 (Cloning host)	−	LB
Gram-positive
Listeria monocytogenes ATCC 19114	++	BHI
Listeria monocytogenes KCTC 3710	++	BHI
Listeria grayi KCTC 3433	++	BHI
Listeria innocua KCTC 3586	++	BHI
Listeria welshimeri KCTC 3587	++	BHI
Enterococcus faecium KCTC 13225	+	BHI
Enterococcus faecalis KCTC 3206	+	BHI
Staphylococcus aureus KFRI 00188	−	NB
Bacillus licheniformis KCCM 12145	−	NB
Bacillus cereus KCTC1661	−	LB
Lactobacillus mucosae LM1	−	MRS
Lactobacillus plantarum SK156	−	MRS
Lactobacillus johnsonii PF01	−	MRS
Lactococcus garvieae KCTC 5618	−	MRS
Ligilactobacillus agilis KCTC 3158	−	MRS
Fungi
Aspergillus flavus KCTC6901	−	PDA
Saccharomyces cerevisiae KCCM7919	−	YM

Antimicrobial activity was measured by agar well diffusion. Inhibition zone was expressed in degrees: −, no inhibition; +, up to 1 mm; ++, up to 5 mm.
KCCM, Korean Culture Center of Microorganisms; NB, nutrient broth (BD, Franklin Lakes, NJ, USA); KVCC, Korea Veterinary Culture Collection; LB, Luria-Bertani (BD); ATCC, American Type Culture Collection; BHI, brain heart infusion (BD); KCTC, Korean Collection for Type Culture; KFRI, Kerala Forest Research Institution; MRS, de Man Rogosa and Sharpe (BD); PDA, potato dextrose agar (BD); YM, yeast alt (BD).