Table 1. Real-time PCR primers and condition used in this study

Gene name	Primer sequences (5’-3’)	Annealing (°C)	Gene accession no.
Myogenic factor 5 (MYF5)	Forward: GTGAGTTCGGGGACGAGTTTReverse: CAGCCTCTGGTTAGGGTTGG	59	NM_174116
Peroxisome proliferator activated receptor gamma (PPARγ)	Forward: ACTTTGGGATCAGCTCCGTGReverse: TCCTCATAGTGCGGAGTGGA	59	NM_181024
Myogenic differentiation 1 (MYOD1)	Forward: ACCCTCAGTGCTTTGCTATCTCReverse: AGTCGAAACACGGGTCATCA	59	NM_001040478
Myogenin (MYOG)	Forward: AACTGAGATTGTCTGCCGGGReverse: CAGAGGGCTTACACAAGGGG	59	NM_001111325
Myogenic factor 6 (MRF4)	Forward: CTTGAGGGTGCGGATTTCCTReverse: CGTCCGGACCACTGAGTTAC	59	NM_181811.2
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	Forward: CTGGAATGAGGAACCTCCGGReverse: GTCAGGAGATTCTCAGTGTGGC	59	NM_001034034

PCR, polymerase chain reaction.