Table 3. Effects of pink inhibiting ingredients without added STP, cooking rate1), and storage day2) on Mb content, total pigments, PMD, rNIC, and nitrosyl hemochrome in cooked ground turkey breast
| Main effects | Mb (uncooked, mg/g) | Total pigments (ppm) | PMD (%) | rNIC | Nitrosyl hemochrome (ppm) |
| Slow | Fast | Day 1 | Day 14 |
| Treatment (T)3) |
| Control | 0.762a | 18.24d | 84.3bcx | 85.9bcx | 1.026bx | 1.004aby | 0.38cd |
| CA 0.1% | 0.753ab | 22.12c | 86.2abx | 86.6abcx | 1.012dx | 1.001by | 0.40bcd |
| CA 0.2% | 0.708ab | 25.22b | 84.9bcy | 87.3abx | 1.003ex | 0.998cy | 0.46abcd |
| CA 0.3% | 0.598c | 27.97a | 81.7dy | 86.2bcx | 0.993fx | 0.991dx | 0.52ab |
| CC 0.025% | 0.738ab | 18.20d | 83.9cx | 85.3cx | 1.021cx | 1.003by | 0.35d |
| CC 0.05% | 0.759a | 18.26d | 83.6cy | 86.0bcx | 1.020cx | 1.004aby | 0.38cd |
| EDTA 0.005% | 0.696b | 22.66c | 87.2ax | 86.0bcx | 1.027abx | 1.006aby | 0.49abc |
| EDTA 0.01% | 0.762a | 23.09c | 87.2ax | 87.9ax | 1.020cx | 1.005aby | 0.53a |
| SC 0.5% | 0.744ab | 22.28c | 84.9bcy | 86.9abcx | 1.027abx | 1.006aby | 0.42abcd |
| SC 1.0 % | 0.695b | 22.32c | 84.2cy | 86.8abcx | 1.031ax | 1.008ay | 0.36d |
| (SEM) | (0.057) | (0.83) | (1.57) | | (0.002) | | (0.05) |
Cooking rate: The samples were cooked by loading the tubes into a 50°C water bath and then immediately setting the water bath to 90°C (slow cooking) or cooked to 76.7°C in a 90°C water bath (fast cooking).
Storage day: Samples were stored (2°C to 3°C) in the dark before color was determined.
Treatment: Ground meat was salted (2% NaCl) and stored for 6 days before pink inhibiting ingredients (PII: Control=no added PII; CA, CC, EDTA, SC) were incorporated, and then stored overnight prior to cooking.
Means within a column with unlike superscript letters are different (p<0.05). Significant treatment by cooking rate interaction for PMD (p<0.05). Significant treatment by storage day interaction for rNIC (p<0.0001).
Means under PMD and rNIC interaction effects within a row with unlike superscript letters are different (p<0.05).
STP, sodium tripolyphosphate; Mb, amount of undenatured myoglobin; PMD, percentage myoglobin denaturation; rNIC, reflectance estimator of nicotinamide hemochrome (%R537 nm/%R553 nm); CA, citric acid; CC, calcium chloride; EDTA, ethylenedinitrilotetraacetic acid disodium salt; SC, sodium citrate; PII, pink inhibiting ligands.