Table 2. Antimicrobial effect of calamansi pulp extracts against five food pathogens by paper disc diffusion assay (unit: mm)

Plant extract	Concentration (mg/disc)												Streptomycin
	1.25		SEM	2.5		SEM	5		SEM	10		SEM
	Day 3	Day 6	SEM	Day 3	Day 6	SEM	Day 3	Day 6	SEM	Day 3	Day 6	SEM
Escherichia coli													17.14 (0.01 mg/disc)
50	ND	ND	-	9.39^a	8.50^Ca	0.314	12.05^Ba	11.67^a	0.158	14.48^a	13.83^Bb	0.121
70	ND	ND	-	8.50^b	9.00^Ba	0.000	12.50^Aa	12.00^b	0.000	14.83^a	14.17^Bb	0.167
90	ND	ND	-	9.50^b	10.06^Aa	0.008	11.50^Cb	11.63^a	0.005	14.33^b	15.05^Aa	0.137
SEM	-	-		0.256	0.007		0.086	0.096		0.138	0.148
Staphylococcus aureus													14.00 (0.01 mg/disc)
50	ND	ND	-	ND	ND	-	10.00^Aa	9.67^Ba	0.118	13.33^a	13.50^Aba	0.118
70	ND	ND	-	ND	ND	-	9.17^Ba	9.33^Ba	0.264	13.00^a	12.83^Ba	0.118
90	ND	ND	-	ND^b	10.05^a	0.002	10.00^Ab	11.17^Aa	0.026	13.17^b	14.15^Aa	0.193
SEM	-	-		-	0.002		0.096	0.216		0.136	0.157
Salmonella Enteritidis													11.06 (0.2 mg/disc)
50	ND	ND	-	9.50^Bb	10.00^Ba	0.000	11.17^a	11.50^Ba	0.118	14.00^Ba	13.00^Bb	0.000
70	ND	ND	-	10.17^Aa	9.00^Cb	0.118	11.33^a	10.50^Cb	0.118	15.00^Aa	12.50^Cb	0.000
90	ND	ND	-	9.67^ABb	10.37^Aa	0.118	11.83^b	12.90^Aa	0.128	15.00^Ab	17.49^Aa	0.067
SEM	-	-		0.136	0.009		0.167	0.042		0.000	0.055
Pseudomonas aeruginosa													19.69 (0.05 mg/disc)
50	ND	ND	-	ND	ND	-	10.16^Ba	8.99^Cb	0.043	12.88^Ba	12.23^Cb	0.140
70	ND	ND	-	ND	ND	-	9.53^Cb	9.77^Ba	0.053	12.91^ABa	12.61^Ba	0.079
90	ND	ND	-	ND	ND	-	10.38^Aa	10.45^Aa	0.012	13.43^Aa	13.52^Aa	0.042
SEM	-	-		-	-		0.028	0.049		0.125	0.053
Listeria monocytogenes													16.16 (0.01 mg/disc)
50	ND	ND	-	ND	ND	-	12.00^Aa	11.17^Bb	0.118	18.50^Aa	16.17^Cb	0.118
70	ND	ND	-	ND	ND	-	10.67^Bb	11.50^Ba	0.118	14.00^Cb	16.83^Ba	0.118
90	ND	ND	-	ND	ND	-	11.00^Bb	12.65^Aa	0.053	17.17^Bb	18.53^Aa	0.136
SEM	-	-		-	-		0.096	0.106		0.096	0.147

The diameter of paper disc (8 mm) is included.
Means within a column with different superscript differ significantly at p<0.05.
Means within extraction period with different superscript differ significantly at p<0.05.
ND, not detected.