Table 3. Assignment of 1H- nuclear magnetic resonance chemical shifts of fraction EGCII 1-3 and 1-4 separated from EGC (EGCII-1)
Reporter group | Residue | Chemical shifts, δ (coupling constants, Hz) |
EGCII 1-3-1 | EGCII 1-3-2 | EGCII 1-4-1 | EGCII 1-4-2 | EGCII 1-4-3 |
H-1 | Glc α | 5.221 (4.0) | - | 5.224 (3.8) | 5.224 (3.8) | - |
GlcNAc α | - | 5.196 (2.6) | - | - | 5.199 (2.6) |
Glc β | 4.663 (8.1) | - | 4.669 (8.0) | 4.664 (8.1) | - |
GlcNAc β | - | 4.749 (7.7) | - | - | - |
Galβ4 | 4.531 (7.9) | 4.447 (7.9) | 4.427 (7.9) | 4.535 (7.9) | 4.446 (7.9) |
| - | 4.451 (7.8) | - | - | 4.452 (7.9) |
H-3ax | Neu5Ac(α2-3) | 1.799(12.11), −12.12)) | - | - | - | - |
Neu5Ac(α2-6) | - | 1.719(12.11), −12.32)) | 1.745(12.31), −12.22)) | - | - |
Neu5Gc(α2-3) | - | - | - | 1.817(12.01), −12.32)) | - |
Neu5Gc(α2-6) | - | - | - | - | 1.745(12.31), −12.22)) |
H-3eq | Neu5Ac(α2-3) | 2.755 (4.43)) | - | - | - | - |
Neu5Ac(α2-6) | - | 2.667 (4.63)) | 2.712 (4.83)) | - | - |
Neu5Gc(α2-3) | - | - | - | 2.774 (4.93)) | - |
Neu5Gc(α2-6) | - | - | - | - | 2.686 (4.63)) |
NAc | Neu5Ac(α2-3) | 2.027 | - | - | - | - |
Neu5Ac(α2-6) | - | 2.028 | 2.028 | - | - |
GlcNAc | - | 2.063 | - | - | - |
NGc | Neu5Gc(α2-3) | - | - | - | 4.115 | - |
Neu5Gc(α2-6) | - | - | - | - | 4.119 |
J3ax, 4,
J3ax, 3eq,
J3eq, 4.
EGC, Etawah Grade colostrum.