Table 3. Assignment of 1H- nuclear magnetic resonance chemical shifts of fraction EGCII 1-3 and 1-4 separated from EGC (EGCII-1)

Reporter group Residue Chemical shifts, δ (coupling constants, Hz)
EGCII 1-3-1 EGCII 1-3-2 EGCII 1-4-1 EGCII 1-4-2 EGCII 1-4-3
H-1 Glc α 5.221 (4.0) - 5.224 (3.8) 5.224 (3.8) -
GlcNAc α - 5.196 (2.6) - - 5.199 (2.6)
Glc β 4.663 (8.1) - 4.669 (8.0) 4.664 (8.1) -
GlcNAc β - 4.749 (7.7) - - -
Galβ4 4.531 (7.9) 4.447 (7.9) 4.427 (7.9) 4.535 (7.9) 4.446 (7.9)
- 4.451 (7.8) - - 4.452 (7.9)
H-3ax Neu5Ac(α2-3) 1.799(12.11), −12.12)) - - - -
Neu5Ac(α2-6) - 1.719(12.11), −12.32)) 1.745(12.31), −12.22)) - -
Neu5Gc(α2-3) - - - 1.817(12.01), −12.32)) -
Neu5Gc(α2-6) - - - - 1.745(12.31), −12.22))
H-3eq Neu5Ac(α2-3) 2.755 (4.43)) - - - -
Neu5Ac(α2-6) - 2.667 (4.63)) 2.712 (4.83)) - -
Neu5Gc(α2-3) - - - 2.774 (4.93)) -
Neu5Gc(α2-6) - - - - 2.686 (4.63))
NAc Neu5Ac(α2-3) 2.027 - - - -
Neu5Ac(α2-6) - 2.028 2.028 - -
GlcNAc - 2.063 - - -
NGc Neu5Gc(α2-3) - - - 4.115 -
Neu5Gc(α2-6) - - - - 4.119
J3ax, 4,
J3ax, 3eq,
J3eq, 4.
EGC, Etawah Grade colostrum.