Table 2. Biofilm-formation ability of Kocuria salsicia isolates (KS1–KS9) in nutrient broth (NB) compared with that of Staphylococcus aureus ATCC 29213 at 5°C, 15°C, 25°C, 30°C, 37°C, and 42°C
Strains | Optical density of isolates1) |
Temperature (°C)2) |
5 | 15 | 25 | 30 | 37 | 42 |
NC | 0.069±0.007 | 0.064±0.003 | 0.068±0.005 | 0.075±0.007 | 0.062±0.004 | 0.048±0.001 |
PC | 0.093±0.013a | 0.131±0.002a | 0.089±0.007a | 0.128±0.005a | 0.066±0.002a | 0.080±0.003a |
KS1 | 0.116±0.007b | 0.139±0.002b | 0.082±0.004a | 0.237±0.025b | 0.121±0.008b | 0.097±0.002a |
KS2 | 0.106±0.002a | 0.136±0.002a | 0.073±0.001a | 0.079±0.001c | 0.066±0.002a | 0.072±0.001a |
KS3 | 0.088±0.005a | 0.147±0.001b | 0.293±0.050b | 0.361±0.003b | 0.133±0.003b | 0.148±0.010b |
KS4 | 0.109±0.008a | 0.132±0.001a | 0.093±0.002a | 0.102±0.008a | 0.083±0.002a | 0.070±0.001a |
KS5 | 0.120±0.010b | 0.142±0.003b | 0.080±0.003a | 0.089±0.005c | 0.065±0.002a | 0.072±0.001a |
KS6 | 0.113±0.001b | 0.187±0.004b | 0.250±0.001b | 0.315±0.011b | 0.132±0.007b | 0.146±0.023b |
KS7 | 0.102±0.003a | 0.129±0.001a | 0.118±0.003a | 0.100±0.005a | 0.078±0.003a | 0.069±0.001a |
KS8 | 0.090±0.002a | 0.137±0.002a | 0.260±0.027b | 0.371±0.003b | 0.183±0.011b | 0.118±0.008b |
KS9 | 0.082±0.001a | 0.137±0.001a | 0.075±0.002a | 0.079±0.003c | 0.136±0.011b | 0.102±0.005a |
Optical density of isolates is expressed as mean±SD.
Biofilm formation by temperature (°C).
Different letters indicate statistical difference at p<0.05 compared to S. aureus ATCC 29213 (Tukey method).
ATCC, American Type Culture Collection; NC, negative control (NB); PC, positive control (S. aureus ATCC 29213+NB); KS1-KS9, K. salsicia KS1-KS9+NB.