Table 4. Tenderness-related traits of samgyetang breast meat marinated with reduced NaCl with CaCl₂ prior to cooking in different manufacturing method

Variable	Cooking method	NaCl replacement percentage¹⁾					SEM	Sample²⁾	Method³⁾	Sample× method⁴⁾
Variable	Cooking method	0%	25%	50%	75%	100%	SEM	Sample²⁾	Method³⁾	Sample× method⁴⁾
Shear force value (kgf)	Boiling	2.30^ab	2.46^a	2.35^ab	2.24^ab	2.19^b	0.03	<0.001	0.16	0.97
Shear force value (kgf)	Retorting	2.28^ab	2.39^a	2.27^ab	2.20^b	2.16^b	0.02	<0.001	0.16	0.97
MFI	Boiling	29.33^cy	30.33^bcy	32.33^by	35.00^ay	36.66^ay	0.59	<0.001	<0.001	0.5
MFI	Retorting	36.00^cx	37.34^bcx	41.66^abx	43.67^ax	41.67^abx	0.90	<0.001	<0.001	0.5
Total collagen (mg/g)	Boiling	1.72	1.58	1.67	1.64	1.78	0.02	0.49	0.93	0.33
Total collagen (mg/g)	Retorting	1.68	1.63	1.75	1.69	1.62	0.03	0.49	0.93	0.33
Insoluble collagen (mg/g)	Boiling	0.79	0.76	0.77	0.77	0.79	0.00	0.1	0.45	0.11
Insoluble collagen (mg/g)	Retorting	0.80	0.77	0.79	0.77	0.77	0.00	0.1	0.45	0.11
Total soluble protein (mg/mL)	Boiling	91.19^cy	91.97^cy	93.20^cy	97.02^by	101.63^ay	0.89	<0.001	<0.001	0.71
Total soluble protein (mg/mL)	Retorting	103.86^cx	104.79^cx	106.33^cx	111.71^bx	116.33^ax	1.08	<0.001	<0.001	0.71
Myofibrillar protein solubility (mg/mL)	Boiling	17.08^y	17.12^y	17.06^y	17.10^y	17.53^y	0.32	0.69	<0.01	0.94
Myofibrillar protein solubility (mg/mL)	Retorting	18.55^x	17.88^x	17.98^x	18.68^x	19.35^x	0.30	0.69	<0.01	0.94
Sarcoplasmic protein solubility (mg/mL)	Boiling	74.11^cy	74.85^cy	76.14^cy	79.91^by	84.10^ay	0.81	<0.001	<0.001	0.85
Sarcoplasmic protein solubility (mg/mL)	Retorting	85.31^bx	86.91^bx	88.35^bx	93.04^ax	96.98^ax	0.99	<0.001	<0.001	0.85

NaCl replacement percentage with CaCl₂, 0% (breast meat marinated with 100% NaCl), 25% (breast meat marinated with 75% NaCl:25% CaCl₂), 50% (breast meat marinated with 50% NaCl:50% CaCl₂), 75% (breast meat marinated with 25% NaCl:75% CaCl₂), 100% (breast meat marinated with 100% CaCl₂).
Sample, significance value after treatment with different NaCl replacement percentage.
Method, significance value after treatment with different manufacturing method.
Sample×method, the interaction between different NaCl replacement percentage with different manufacturing method.
Mean values within the same row indicating a significant different following NaCl replacement with CaCl₂.
Mean values within the same column indicating a significant different following different manufacturing method.
MFI, myofibril fragmentation index.