Table 4. Production of diacetyl with the addition of different concentrations of citrate
Citrate (%, w/v) | Diacetyl (mg/L) |
0 | 19.85±0.38 |
0.1 | 40.48±0.32b |
0.2 | 43.26±0.44b |
0.3 | 42.75±1.41b |
0.5 | 43.01±0.59b |
1.0 | 42.85±1.43b |
2.0 | 34.43±1.64a |
3.0 | 35.20±1.05a |
Results are expressed as mean±SE (n=3).
MRS broth was used as the basal medium, citrate was added before sterilization (autoclave, 121°C, 15 min). The initial concentration of diacetyl was 0.
Means in the same column with different lowercase superscript letters are significantly different at p<0.05.