Table 1. Primer sequences of real-time PCR for gene amplification

Tissue	Gene	Forward (5'→3')	Reverse (5'→3')
Liver	HMG CoA R	TCC AGT TCC AGA ACC TAC GG	ACA AGG CAT TCC ACA AGA GC
	CYP7A1	CAA GAA CCT GTA CAT GAG GGA C	CAC TTC TTC AGA GGC TGC TTT C
Gallbladder	MUC1	CCA CAG TAG TGC CTC CAT CC	GCC ATG GTA GGA GAA ACA GG
	MUC2	CTT CCA ACC CTC CTC CTA CC	GCG TCT CTG ACC TCT TCA GG
	MUC5AC	TGA GAG ATG CCT GTG TGA GG	AGC ATC CGT CTT CTC TCA GC
	MUC5B	ATC GAT GAG TGC AAC TGT GC	GAG AAT GAG GCC AAA ACA GC
β-Actin		CCT CTA TGC CAA CAC AGT	AGC CAC CAA TCC ACA CAG

HMG CoA R, 3-hydroxy-3-methylglutaryl coenzyme A reductase; CYP7A1, cholesterol 7α-hydroxylase; MUC, mucin.