Table 2. Amino acid profile and essential amino acid index (EAAI) of edible insect protein extracted at different pressure levels

	High pressure (MPa)						FAO/WHO/UNU (1985)
	Control1)	100	200	300	400	500
Amino acid profile (mg/g)
Essential amino acid (EAA)
His	7.80±0.28	6.25±0.07	7.65±0.49	7.30±0.28	7.05±0.35	7.45±0.64	15
Ile	10.60±0.14b	9.45±0.21c	11.75±0.07a	11.25±0.49ab	11.65±0.21a	11.50±0.57a	30
Leu	18.25±0.35ab	16.05±0.49c	19.20±0.57a	17.55±1.06b	19.25±0.49a	18.50±0.42ab	59
Lys	12.95±0.07bc	10.30±0.28d	14.00±0.14a	12.80±0.28c	13.35±0.21b	12.75±0.21c	45
Met+Cys	1.35±0.21	1.55±0.35	1.55±0.07	1.55±0.21	1.65±0.49	2.30±0.14	22
Phe+Tyr	27.75±0.49c	23.45±0.35d	28.70±0.99c	27.45±1.91c	35.65±1.06a	31.70±0.85b	38
Thr	9.00±0.21b	7.40±0.14c	9.80±0.28a	8.90±0.00b	9.65±0.21a	9.20±0.14b	23
Val	8.75±0.21b	7.50±0.14c	9.70±0.28a	8.75±0.07b	9.50±0.28ab	9.60±0.57a	39
Sum of EAA	96.45±1.06c	81.95±0.35d	102.35±1.63b	95.55±0.07c	107.75±0.92a	103.00±3.25b	271
Ala	12.85±0.07b	10.50±0.14e	13.20±0.00a	12.00±0.14d	12.40±0.00c	12.35±0.21c
Arg	9.75±0.21a	7.10±0.99c	9.30±0.57ab	8.00±0.71bc	9.40±0.00ab	8.55±0.35abc
Asp	16.50±0.14ab	12.90±0.00d	17.30±0.14a	15.30±0.42c	17.20±0.57ab	16.35±0.49b
Glu	29.10±0.14c	23.35±0.21d	31.80±0.28a	28.25±0.64c	31.75±0.21a	30.30±0.57b
Pro	22.10±0.57a	15.10±0.57c	21.20±1.41ab	13.70±0.85c	19.00±1.13b	18.35±1.77b
Gly	11.85±0.07c	9.65±0.21e	12.40±0.00a	11.30±0.00d	12.20±0.00ab	12.10±0.14bc
Ser	12.60±0.28b	10.55±0.35c	13.55±0.21a	12.35±0.35b	13.65±0.21a	12.55±0.07b
Sum of total AA	114.75±0.21b	89.15±0.64e	118.75±1.48a	100.90±3.11d	115.60±1.70b	110.55±1.20c
EAAI	15.16±0.29b	13.60±0.21c	16.05±0.07a	15.24±0.08b	16.20±0.51a	16.45±0.50a

All values are mean±SD of three replicates (n=3).
Means within a row with different letters are significantly different (p<0.05).
P. brevitarsis seulensis was high hydrostatic pressured at 0 (control), 100, 200, 300, 400, and 500 MPa.
EAAI, essential amino acid index.