Table 1. Summary of the seven commercial master mixes evaluated in this study

Manufacturer	Master mix	Manufacturer’s recommended thermal cycling conditions
Applied Biosystems	TaqMan Universal PCR Master Mix	50°C for 2 min, 95°C for 10 min, and 35 cycles of 95°C for 15 s and 60°C for 1 min
CancerROP	MG 2X qPCR MasterMix (TaqMan) with ROX	95°C for 5 min and 35 cycles of 95°C for 30 s and 60°C for 1 min
Invitrogen	Express qPCR Supermix Universal	50°C for 2 min, 95°C for 2 min, and 35 cycles of 95°C for 15 s and 60°C for 1 min
Kogene Biotech	PowerAmp Real-time PCR Master Mix II	50°C for 2 min, 95°C for 10 min, and 35 cycles of 95°C for 15 s and 60°C for 1 min
New England Biolabs	Luna Universal Probe qPCR Master Mix	95°C for 60 s and 35 cycles of 95°C for 15 s and 60°C for 30 s
Qiagen	QuantiNova Probe PCR Kit	95°C for 2 min and 35 cycles of 95°C for 5 s and 60°C for 10 s
Takara	Premix Ex Taq (Probe qPCR), ROX plus	95°C for 20 s and 35 cycles of 95°C for 1 s and 60°C for 20 s