Table 1. Combinations of the mobile phase and the corresponding elution time for UHPLC

A (%)	B (%)	Time (min)
65	35	0
45	55	1.5
15	85	3.5
65	35	3.5
65	35	4.0

A (1 mM ammonium acetate) and B (methyl alcohol) are solutions making up the moblie phase.
UHPLC, ultra high performance liquid chromatography.