| 4 | DNA N-6-adenine-methyltransferase[Escherichia phage vB_EcoS_ESCO41] | Replication and regulation | 479 | 92.98 | AQY55313.1 |
| 7 | Helicase [Enterobacteria phage vB_EcoS_NBD2] | Replication and regulation | 1,072 | 73.69 | YP_009284684.1 |
| 9 | DNA primase/helicase [Escherichia phage vB_EcoS-IME253] | Replication and regulation | 588 | 92.16 | APU93271.1 |
| 10 | Tail fiber protein [Enterobacter cloacae] | Structure | 539 | 60.95 | SAD85978.1 |
| 12 | ERF superfamily protein [Escherichia phage vB_EcoS_ESCO41] | Replication and regulation | 426 | 94.04 | AQY55302.1 |
| 14 | gp32 [Escherichia phage EB49] | Replication and regulation | 147 | 96.05 | YP_009018646.1 |
| 17 | Tail assembly protein I [Escherichia phage vB_EcoS_ESCO41] | Replication and regulation | 388 | 97.46 | AQY55296.1 |
| 18 | NlpC/P60 family protein [Escherichia phage vB_EcoS_ESCO41] | Cell lysis | 486 | 94.61 | AQY55295.1 |
| 19 | Minor tail protein [Escherichia phage vB_EcoS_ESCO41] | Structure | 486 | 91.94 | AQY55294.1 |
| 20 | Minor tail protein [Escherichia phage vB_EcoS_ESCO41] | Structure | 238 | 99.13 | AQY55292.1 |
| 21 | Tail length tape measure protein[Escherichia phage vB_EcoS_ESCO41] | Replication and regulation | 1,108 | 60.30 | AQY55291.1 |
| 23 | Tape measure chaperone [Escherichia phage SRT8] | Replication and regulation | 138 | 61.17 | YP_009615420.1 |
| 24 | Tail protein [Escherichia phage vB_EcoS_ESCO41] | Structure | 459 | 99.55 | AQY55289.1 |
| 33 | AP2 domain protein [Escherichia phage vB_EcoS_ESCO41] | Replication and regulation | 289 | 85.62 | AQY55280.1 |
| 36 | HNH homing endonuclease[Enterobacteria phage vB_EcoS_IME542] | Replication and regulation | 182 | 55.95 | QAU04414.1 |
| 37 | Terminase-like family protein[Escherichia phage vB_EcoS_ESCO41] | DNA packaging | 1,025 | 92.59 | AQY55276.1 |
| 54 | DUF600 family protein [Endomicrobium proavitum] | Additional function | 35.8 | 39.53 | WP_052570750.1 |
| 64 | Coiled-coil and C2 domain-containing protein 1-like isoform X2[Scaptodrosophila lebanonensis] | Replication and regulation | 36.6 | 37.29 | XP_030384370.1 |
| 76 | Putative lysozyme [Escherichia phage Henu8] | Cell lysis | 259 | 71.60 | QEA10109.1 |
| 77 | Putative holin [Enterobacteria phage vB_EcoS_NBD2] | Cell lysis | 113 | 73.24 | YP_009284693.1 |