Table 1. Primers, probes, and internal amplification control sequence used for Salmonella spp.-specific real-time PCR

Designation	Sequence	Positions1)
ttr-6 (forward)	CTCACCAGGAGATTACAACATGG	4287–4309
ttr-4 (reverse)	AGCTCAGACCAAAAGTGACCATC	4359–4381
ttr-5 (target probe)	FAM-CACCGACGGCGAGACCGACTTT-TAMRA
IAC sequence2)	CGTCAGTGTGAAGCGGTTATAAATCTGCTCTTTCGCGGTATCCGTACCGATTTCGGTAAGGTAAACCCCGTTTTTGTTTCGCTTACGTGGCAT
IAC (forward)	CGTCAGTGTGAAGCGGTTATAA
IAC (reverse)	ATGCCACGTAAGCGAAACA
IAC probe	HEX-TGCTCTTTCGCGGTATCCGTACCGAT-TAMRA

Positions correspond to Genbank accession no. AF282268.
The sequences marked in boldface type are the primer binding sites for IAC, and the underlined sequence is the reverse complement IAC probe binding site.
IAC, internal amplification control.